小麦抗叶锈病基因近等基因系TcLr35基因表达差异研究

被引：6

作者：

陈云芳

杨文香

闫红飞

刘力强

褚栋

刘大群

机构：

[1] 河北农业大学植物保护学院河北省农作物病虫害生物防治工程技术研究中心

来源：

植物病理学报 | 2007年 / 01期

关键词：

genes expression; cDNA-AFLP; resistance gene; TcLr35;

D O I：

10.13926/j.cnki.apps.2007.01.019

中图分类号：

S512.1 [小麦];

学科分类号：

0901 ;

摘要：

The differential genes expression of adult plant resistance gene of TcLr35 was analysed by using cDNA-AFLP technique.The results showed that 33 of 843 amplified bands were differential ones in different growth stages of TcLr35.A polymorphic band amplified by primer pair E-TG/M-CTG was found at different leaf-growing stages from the second to the sixth leaf of TcLr35 and absent in Thatcher and the seedling stage of TcLr35 on TcLr35 without inoculated with Puccinia recondita.Reverse Northern blotting test suggested that band E-TG/M-CTG-192 was a piece of special gene fragment of TcLr35.The band was cloned and sequenced subsequently.The fragment of 192 bp produced by E-TG/M-CTG contained an open reading frame(ORF),but did not show homologous to the published wheat gene sequences.

引用

页码：109 / 112

页数：4

共 4 条

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[2]

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[3]

Kolmer J A.Virulence inPuccinia reconditafsp.triticiisolates from Canada to genes for adult plant re-sistance to wheat leaf rust. Plant Disease . 1997

[4]

Manninger K.Effective resistance genes as sources ofresistance against Hungarian wheat rusts. CzechJournal Genetic Plant Breed . 2002

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