银杏二萜内酯葡胺注射液通过下调calpain信号通路抑制脑缺血神经细胞凋亡

被引：21

作者：

刘秋

许治良

金治全

周军

毕宇安

王振中

萧伟

机构：

[1] 江苏康缘药业股份有限公司中药制药过程新技术国家重点实验室

来源：

中国药科大学学报 | 2015年 / 46卷 / 06期

关键词：

银杏二萜内酯葡胺注射液; 缺氧缺糖损伤; 细胞凋亡; 钙离子; calpain; caspase-12;

D O I：

暂无

中图分类号：

R285 [中药药理学];

学科分类号：

摘要：

研究银杏二萜内酯葡胺注射液（diterpene ginkgolides meglumine injection DGMI）对缺氧缺糖/复氧（oxygen-glucose deprivation/reoxygenation,OGD/R）损伤的人神经母细胞瘤细胞SH-SY5Y凋亡的抑制作用及其机制。采用试剂盒、Fluo-3 AM法、Western blot检测SH-SY5Y细胞氧糖剥离损伤4h后,与药物一起复氧1h细胞乳酸脱氢酶（LDH）的漏出量、caspase-3/7酶活力、细胞质中核小体含量、胞浆游离钙离子浓度以及calpain、cleaved caspase-12蛋白量的变化。结果显示DGMI能极显著降低OGD/R损伤的SH-SY5Y细胞LDH的漏出量,抑制caspase-3/7酶活性,减少细胞核核小体的释放量,下调细胞内游离钙离子浓度、calpain和cleaved caspase-12蛋白量,抑制calpain凋亡信号通路保护神经细胞。DGMI对OGD/R诱导的SH-SY5Y细胞凋亡具有显著的抑制作用,其作用机制可能与抑制细胞内Ca2+/calpain/caspase-12/capase-3信号通路有关。

引用

页码：707 / 711

页数：5

共 6 条

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