西格列汀对脂多糖诱导胰岛β细胞数量与功能改变的影响

被引：6

作者：

胡星云 ^{[1
]}

刘珊英 ^{[2
]}

刘晓丹 ^{[1
]}

蒋清凌 ^{[1
]}

严励 ^{[1
]}

李焱 ^{[1
]}

机构：

[1] 广州, 中山大学孙逸仙纪念医院内分泌科

[2] 中山大学孙逸仙纪念医院林百欣医学研究中心

来源：

中华内分泌代谢杂志 | 2015年 / 31卷 / 05期

关键词：

二肽基肽酶Ⅳ抑制剂; 脂多糖; 胰岛β细胞;

D O I：

暂无

中图分类号：

R965 [实验药理学];

学科分类号：

100602 ; 100706 ;

摘要：

目的探讨二肽基肽酶Ⅳ(DPP-4)抑制剂对脂多糖诱导的胰岛β细胞数量与功能改变的影响。方法不同浓度西格列汀(Sitagliptin)与脂多糖处理RINm细胞24 h后, 采用CCK-8比色法检测细胞增殖, Annexin V/PI染色流式细胞术检测细胞凋亡, ELISA法检测细胞胰岛素分泌功能, RT-PCR检测白细胞介素6(IL-6)mRNA表达。结果脂多糖组较对照组明显促进RINm细胞的增殖(0.89±0.04对1.14±0.08, P<0.01), 但脂多糖+西格列汀各组与脂多糖组相比差异均无统计学意义;脂多糖+10-1mmol/L西格列汀组凋亡率较脂多糖组明显减低;各组之间胰岛素基础分泌量无明显差异, 但高糖/低糖刺激后, 脂多糖组较对照组胰岛素分泌量增加;脂多糖+西格列汀组IL-6 mRNA表达较脂多糖组明显减低(0.77±0.33对1.30±0.41, P=0.006)。结论 DPP-4抑制剂对脂多糖诱导β细胞增殖的影响较弱, 但一定程度上能够抑制脂多糖对β细胞促凋亡及胰岛素分泌功能的作用, 这可能与炎症因子IL-6的表达有关。

引用

页码：447 / 451

页数：5

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