<正>序列相关扩增多态性标记(sequence related amplified polymorphism,SRAP)是由Li and Quiros(2001)发展的一种新的分子标记技术。在园艺作物研究中,Riazetal.(2004)发现SRAP标记在鉴定区分桃和油桃品种时比SSR标记更有效;Ruizetal.(2005)发现可利用SRAP检测出番茄品种内的变异,而SSR标记则无法检测出来。迄今还没有SRAP标记技术在梨树上应用的研究报道,本研究以梨为研究材料,建立并优化了SRAP反应体系。
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[1]
Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica[J] . G. Li,C. F. Quiros.TAG Theoretical and Applied Genetics . 2001 (2-3)
[1]
Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica[J] . G. Li,C. F. Quiros.TAG Theoretical and Applied Genetics . 2001 (2-3)