菌细胞脂肪酸气相色谱图鉴别非发酵菌和肠杆菌

被引：2

作者：

卢焱

叶萍

王靖

张永生

凌代文

翟振兴

戴富保

机构：

[1] 第二军医大学长海医院!上海

[2] 第二军医大学药学院

来源：

中华微生物学和免疫学杂志 | 1996年 / 06期

关键词：

非发酵菌; 肠杆菌; 气相色谱; 细胞脂肪酸;

D O I：

暂无

中图分类号：

R446.5 [微生物学检验];

学科分类号：

100208 ;

摘要：

本研究用高效毛细管柱气相色谱（ＧＣ）法分析６属１２种１１８株常见非发酵菌和１１种４０株肠杆菌的全细胞脂肪酸（ＣＦＡｓ），并与Ｖｉｔｅｋ－ＡＭＳ的分析结果进行比较。结果表明：两类菌有鉴别意义的ＣＦＡｓ是葵酸、月桂烯酸、月桂酸、十三碳烯酸、十三碳酸、肉豆蔻脑酸、肉豆蔻酸、十五碳烯酸、十五碳酸、十七碳烯酸、十七碳酸、亚油酸、硬脂酸、花生四烯酸、花生酸和二十一碳酸。非发酵菌和肠杆菌的ＣＦＡ构成差别较大。各菌属间的差别较种间大。同属不同种间的差别小且主要是ＣＦＡｓ百分含量不同。ＧＣ法将为临床微生物学检验提供一种全新而高效的检测方法。

引用

页码：402 / 404

页数：3

共 8 条

[1]

Cellular fatty acids in Fusobacterium species as a tool for identification. Tuner K,Baron EJ,Summanen P,et al. Journal of Clinical Microbiology . 1992

[2]

Gas-liquid chromatography of cellular fatty acids for identification of staphylococci. Stoakes L,John MA,Lannigan R,et al. Journal of Clinical Microbiology . 1994

[3]

Comparison of the fatty acid profiles of Borrelia Ser pulina and Le ptos pira species. Livesley MA,Thompson IP,Bailey MJ,et al. Journal of General Microbiology . 1993

[4]

Comparative study of the cellular fatty acids of methicillin-resistant and-susceptible Staphylococcus aureus. Asai S,Noda M,Yamamura M,et al. Acta Pathologica Microbiologica et Immunologica Scandinavica . 1993

[5]

Identification of clinical isolates of gram-negative nonfermentative bacteria by an automated cellular fatty acid identification system. Osterhout GJ,Shull VH,Dick JD,et al. Journal of Clinical Microbiology . 1991

[6]

Hydroxy-fatty acid profiles of Legionella species: diagnostic usefulness assessed by principal component analysis. Jantzen E. Journal of Clinical Microbiology . 1992

[7]

Gas chromatographic whole-cell fatty acid analysis as an aid for the identification of mixed mycobacterial cultures. Garcia BM,Luquin M,Belda F,et al. Journal of Chromatography . 1993

[8]

Gas chromatographic whole-cell fatty acid analysis as an aid for the identification of mixed mycobacterial cultures. Garcia BM,Luquin M,Belda F,et al. Journal of Chromatography . 1993

← 1 →