人BMSCs自噬水平和成骨分化能力比较

被引：17

作者：

徐丽丽

胥方元

万永鲜

机构：

[1] 西南医科大学附属医院

来源：

中国骨质疏松杂志 | 2018年 / 24卷 / 11期

关键词：

自噬相关蛋白质类; 骨质疏松; 骨髓间充质干细胞; 成骨分化;

D O I：

暂无

中图分类号：

R580 [];

学科分类号：

摘要：

目的比较老年骨质疏松(osteoporosis,OP)和健康成年人椎体中骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)自噬水平和成骨分化能力。方法取从2组手术患者椎体中培养的BMSCs细胞,Western blot检测自噬相关基因LC3和P62蛋白表达。转染含LC3-GFP质粒的腺病毒后,激光共聚焦下观察代表自噬体的绿色荧光斑点数。细胞经成骨诱导分化培养基刺激后,碱性磷酸酶染色比较早期成骨指标,茜素红染色比较晚期成骨指标钙盐沉积的影响。荧光定量PCR(q-PCR)检测骨形成相关基因I(collagen type I,COL I)、骨钙素(osteocalcin,OCN)、骨桥蛋白(osteopontin,OPN)以及Runt相关转录因子2(RUNX2)。结果 Western blot结果显示OP组BMSCs的LC3-II/I表达明显降低,而P62表达明显增高(P<0.05);激光共聚焦观察进一步证实OP组中绿色荧光斑点数目显著下降,说明细胞内自噬体数目减少(P<0.05)。两组BMSCs经成骨诱导后,茜素红和碱性磷酸酶染色发现OP组阳性程度明显弱于正常对照组,而q-PCR结果显示成骨分化的一些关键指标COL I、OCN、OPN和RUNX2在OP组中均显著下降,差异具有统计学意义(P<0.05)。结论骨质疏松的BMSCs中自噬水平较正常细胞低,且成骨分化能力更弱。

引用

页码：1446 / 1450+1463 +1463

页数：6

共 10 条

[1]

Hongjingtian Injection Attenuates Myocardial Oxidative Damage via Promoting Autophagy and Inhibiting Apoptosis.[J].Shujing Zhang;Ling Zhang;Han Zhang;Guanwei Fan;Jiuwen Qiu;Zongbao Fang;Haibo Wu;Yi Wang;Xiaoping Zhao;Zhenquan Jia.Oxidative Medicine and Cellular Longevity.2017,

[2]

Staphylococcal lipoteichoic acid promotes osteogenic differentiation of mouse mesenchymal stem cells by increasing autophagic activity [J].

Liu, Xin ;

Wang, Yuan ;

Cao, Zhen ;

Dou, Ce ;

Bai, Yun ;

Liu, Chuan ;

Dong, Shiwu ;

Fei, Jun .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2017, 485 (02) :421-426

[3]

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition) [J].

Klionsky, Daniel J. ;

Abdelmohsen, Kotb ;

Abe, Akihisa ;

Abedin, Md Joynal ;

Abeliovich, Hagai ;

Arozena, Abraham Acevedo ;

Adachi, Hiroaki ;

Adams, Christopher M. ;

Adams, Peter D. ;

Adeli, Khosrow ;

Adhihetty, Peter J. ;

Adler, Sharon G. ;

Agam, Galila ;

Agarwal, Rajesh ;

Aghi, Manish K. ;

Agnello, Maria ;

Agostinis, Patrizia ;

Aguilar, Patricia V. ;

Aguirre-Ghiso, Julio ;

Airoldi, Edoardo M. ;

Ait-Si-Ali, Slimane ;

Akematsu, Takahiko ;

Akporiaye, Emmanuel T. ;

Al-Rubeai, Mohamed ;

Albaiceta, Guillermo M. ;

Albanese, Chris ;

Albani, Diego ;

Albert, Matthew L. ;

Aldudo, Jesus ;

Alguel, Hana ;

Alirezaei, Mehrdad ;

Alloza, Iraide ;

Almasan, Alexandru ;

Almonte-Beceril, Maylin ;

Alnemri, Emad S. ;

Alonso, Covadonga ;

Altan-Bonnet, Nihal ;

Altieri, Dario C. ;

Alvarez, Silvia ;

Alvarez-Erviti, Lydia ;

Alves, Sandro ;

Amadoro, Giuseppina ;

Amano, Atsuo ;

Amantini, Consuelo ;

Ambrosio, Santiago ;

Amelio, Ivano ;

Amer, Amal O. ;

Amessou, Mohamed ;

Amon, Angelika ;

An, Zhenyi .

AUTOPHAGY, 2016, 12 (01) :1-222

[4]

Human mesenchymal stem cells/multipotent stromal cells consume accumulated autophagosomes early in differentiation [J].

Nuschke, Austin ;

Rodrigues, Melanie ;

Stolz, Donna B. ;

Chu, Charleen T. ;

Griffith, Linda ;

Wells, Alan .

STEM CELL RESEARCH & THERAPY, 2014, 5

[5]

Autophagy in osteoblasts is involved in mineralization and bone homeostasis [J].

Nollet, Marie ;

Santucci-Darmanin, Sabine ;

Breuil, Veronique ;

Al-Sahlanee, Rasha ;

Cros, Chantal ;

Topi, Majlinda ;

Momier, David ;

Samson, Michel ;

Pagnotta, Sophie ;

Cailleteau, Laurence ;

Battaglia, Severine ;

Farlay, Delphine ;

Dacquin, Romain ;

Barois, Nicolas ;

Jurdic, Pierre ;

Boivin, Georges ;

Heymann, Dominique ;

Lafont, Frank ;

Lu, Shi Shou ;

Dempster, David W. ;

Carle, Georges F. ;

Pierrefite-Carle, Valerie .

AUTOPHAGY, 2014, 10 (11) :1965-1977

[6]

p62 Is Required for Stem Cell/Progenitor Retention through Inhibition of IKK/NF-κB/Ccl4 Signaling at the Bone Marrow Macrophage-Osteoblast Niche [J].

Chang, Kyung Hee ;

Sengupta, Amitava ;

Nayak, Ramesh C. ;

Duran, Angeles ;

Lee, Sang Jun ;

Pratt, Ronald G. ;

Wellendorf, Ashley M. ;

Hill, Sarah E. ;

Watkins, Marcus ;

Gonzalez-Nieto, Daniel ;

Aronow, Bruce J. ;

Starczynowski, Daniel T. ;

Civitelli, Roberto ;

Diaz-Meco, Maria T. ;

Moscat, Jorge ;

Cancelas, Jose A. .

CELL REPORTS, 2014, 9 (06) :2084-2097

[7]

Therapeutic effects of intrabone and systemic mesenchymal stem cell cytotherapy on myeloma bone disease and tumor growth [J].

Li, Xin ;

Ling, Wen ;

Khan, Sharmin ;

Yaccoby, Shmuel .

JOURNAL OF BONE AND MINERAL RESEARCH, 2012, 27 (08) :1635-1648

[8]

Mesenchymal stem cells in health and disease [J].

Uccelli, Antonio ;

Moretta, Lorenzo ;

Pistoia, Vito .

NATURE REVIEWS IMMUNOLOGY, 2008, 8 (09) :726-736

[9]

自噬对骨质疏松症骨代谢的影响研究 [J].

万永鲜 ;

徐丽丽 ;

卓乃强 ;

阳运康 ;

鲁晓波 .

中国骨质疏松杂志, 2018, 24 (04) :552-556

[10]

自噬活性改变对骨髓间充质干细胞成骨分化能力的影响 [J].

李军 ;

王倩 ;

林小星 ;

李炳宗 .

华中科技大学学报(医学版), 2012, 41 (06) :675-679

← 1 →