淡水白鲳细胞建系及其生长温度特性的研究

被引：10

作者：

张铭陈荪红赵小立 ^{[1
]}

机构：

[1] 浙江大学生命科学学院!杭州

[2] 上海第二医科大学!上海

来源：

生物工程学报 | 2001年 / 01期

基金：

浙江省自然科学基金;

关键词：

淡水白鲳; 细胞系; 胚胎; 动物细胞培养;

D O I：

10.13345/j.cjb.2001.01.023

中图分类号：

Q954.633 [];

学科分类号：

071002 ;

摘要：

Four cell lines originated from embryo,caudal fin,anal fin and snout of Colossoma brachypomum were established,and subcultured for 70,47,45 and 40 passages to the present.The results of the experiment indicated that the four cell lines were fibroblast\|like diploid cells,and the model number of their chromosome is 54.These cell lines could grow under temperature of 20-35℃,and its optimum temperature was 27-30℃.The cells grew slowly at 20℃,stopped growth at 15℃,and died below 10℃.This indicated that the growth temperature of the these cell lines was coincident with that of fish.In addition,the attachment time,doubling time,mitotic index,passage survival rate and cell cycle were determined and compared.

引用

页码：105 / 108

页数：4

共 5 条

[1] 牙鲆的组织培养与鳃细胞系的获得 [J].