用逆转录多聚酶链式反应从凡纳对虾中检出传染性皮下组织和造血器官坏死病毒

被引:13
作者
刘荭
高隆英
史秀杰
江育林
机构
[1] 深圳出入境检验检疫局
[2] 深圳出入境检验检疫局 广东深圳
[3] 广东深圳
关键词
传染性皮下组织和造血器官坏死病毒; 凡纳对虾; 逆转录多聚酶链式反应;
D O I
暂无
中图分类号
S945.4 [各种虾的病虫害及其防治];
学科分类号
0906 ;
摘要
A reverse transcription polymerase chain reaction (RT PCR) was used for detection of infectious hypodermal and haematopoietic necrosis virus (IHHNV) from the gills and subcutaneous tissue of Penaeus vannamei (10-15cm in body size) without clinical signs. With primers 77012R and 77353F, a fragment of 356bp of IHHNV was amplified in RT PCR. The amplified product was sequenced and showed more than 98% homologue with the sequences of other IHHNV strains in Gene Bank.
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页码:185 / 188
页数:4
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