镉胁迫对拟南芥幼苗错配修复基因表达的影响

被引：10

作者：

张巧丽

刘宛

李培军

韩艳萍

机构：

[1] 中国科学院沈阳应用生态研究所

来源：

生态学杂志 | 2008年 / 07期

关键词：

Cd; 生物标记物; 错配修复基因; 增殖细胞核抗原; 半定量RT-PCR;

D O I：

10.13292/j.1000-4890.2008.0221

中图分类号：

X173 [环境植物学];

学科分类号：

摘要：

采用半定量反转录聚合酶链式反应(RT-PCR)技术研究了Cd胁迫对拟南芥(Arabidopsis thaliana)幼苗错配修复和增殖细胞核抗原基因表达的影响,并结合幼苗的形态和生理指标,选取Cd胁迫敏感的生物标记物。结果表明:不同浓度(0.25、0.5、1.0 mg.L-1)Cd处理对拟南芥幼苗叶片数、地上部鲜质量影响不大;Cd浓度为0.25 mg.L-1时,地上部分可溶性蛋白质含量明显升高(P<0.05),Cd浓度为0.5和1.0 mg.L-1时,可溶性蛋白质含量明显降低(P<0.05);叶绿素含量随着Cd浓度的增加而微弱增加(P>0.05)。Cd浓度为0.25 mg.L-1时,以18S rRNA为内参照,PCNA1、PCNA2、MSH2、MSH3、MSH6、MSH7 6个基因均出现了诱导表达,当Cd浓度增加到1.0 mg.L-1时,除了MSH6持续表达诱导及MSH3基因与对照相比表达抑制外,其他基因的表达依然出现诱导,但都低于0.5 mg.L-1Cd处理下的基因表达水平。以上结果表明,基因表达的改变可作为检测Cd污染对植物遗传毒性效应潜在有用的生物标记物。

引用

页码：1173 / 1180

页数：8

共 46 条

[1]

Biological response to ionizing radiation in mouse embryo fibroblasts with a targeted disruption of the DNA polymerase beta gene. Miura M,Watanabe H,Okochi K,et al. Radiation Research . 2000

[2]

A multi-biomarker approach to study the effects of smoking on oxidative DNA damage and repair and antioxidative defense mechanisms. Nia AB,Van Schooten FJ,Schilderman PA,et al. Carcinogenesis . 2001

[3]

Epigenetic regulation of the MGMT and hMSH6 DNA repair genes in cells resistant to methylating agents. Bearzatto A,Szadkowski M,Macpherson P,et al. Cancer Research . 2000

[4]

Cadmium inhibits mismatch repair by blocking the ATPase??activity of the MSH2-MSH6 complex. Flores-Rozas H. Nucleic Acids Research . 2005

[5]

The oxidative DNA base damage in tests of rats after intraperitoneal cadmium injection. Atesi I,Suzen HS,Aydin A,et al. Biometals . 2004

[6]

Arabidopsis thaliana,a versatile model systemfor study of eukaryotic genome maintenance functions. Hays JB. DNARepair . 2002

[7]

Toxicity andDNA damage in tobacco and potato plants growing on soilpolluted with heavy metals. Gichner T,Patkova Z,Szakova J,et al. Ecotoxicology and Environmen-tal Safety . 2006

[8]

Arabidopsis MutS Homologs-at-MSH2,atMSH3,atMSH6,and a novel atMSH7-form threedistinct protein heterodimers with different specificities formismatched DNA. Culligan KM,Hays JB. The Plant Cell . 2000

[9]

Cadmium inhibits the functions of eukaryotic MutS complexes. Clark AB,Kunkel TA. Journal of Biological Chemistry . 2004

[10]

DNA damage-related gene expression as biomarkers to assess cellular response after gamma irradiation of a human lymphoblastoid cell line. Bishay K,Ory K. Oncogene 2000 17 .

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