利用ISSR和SSR分子标记构建北柴胡遗传图谱

被引:78
作者
战晴晴 [1 ,2 ]
隋春 [1 ]
魏建和 [1 ]
范圣此 [1 ]
张杰 [2 ]
机构
[1] 中国医学科学院、北京协和医学院药用植物研究所
[2] 东北林业大学生命科学学院
关键词
北柴胡; 遗传图谱; ISSR; SSR;
D O I
暂无
中图分类号
S567.79 [];
学科分类号
摘要
以北柴胡(Bupleurum chinense DC.)种内杂交所得的F1代96株植株为作图群体,利用拟测交理论,进行北柴胡遗传图谱构建。经父母本多态性筛选,从30条ISSR(inter-simple sequence repeat,内部简单重复序列)和44对SSR(simple sequence repeat,简单重复序列)引物中筛选出28条ISSR和14对SSR多态性引物。对F1代进行基因型和连锁分析,初步构建了包含13个连锁群、80个(72个ISSR和8个SSR)位点的首张北柴胡遗传图谱。该图谱覆盖长度2633.9cM,平均图距33.4cM,13个连锁群包含2~31个标记不等,连锁群遗传距离15.4~1295.7cM。该图谱为北柴胡性状基因定位、图位克隆以及分子标记辅助选择育种等研究奠定了基础。
引用
收藏
页码:517 / 523
页数:7
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