兔骨髓间充质干细胞的分离培养及其生物学性状的研究

被引：2

作者：

杨自权 ^{[1
]}

卫小春 ^{[1
]}

郝一勇 ^{[2
]}

焦强 ^{[1
]}

丁娟 ^{[1
]}

李鹏翠 ^{[1
]}

陈崇伟 ^{[1
]}

机构：

[1] 山西医科大学第二医院骨科

[2] 太原钢铁总公司医院骨科

来源：

中国骨伤 | 2004年 / 05期

关键词：

骨髓; 间充质干细胞; 细胞培养; 生物学性状;

D O I：

暂无

中图分类号：

R329-33 [组织学技术];

学科分类号：

100101 ;

摘要：

目的 :分离培养兔骨髓间充质干细胞 ,对其生物学性状进行观察。方法 :用密度梯度离心法与贴壁培养法相结合 ,分离兔骨髓间充质干细胞 ,进行体外培养扩增 ,绘制其生长曲线 ,用倒置显微镜、细胞爬片、扫描电镜、透射电镜观察细胞的生物学性状。结果 :密度梯度离心后 ,活细胞比例在 95 %以上 ,贴壁培养的细胞呈纺锤形 ,细胞增殖力强 ,平均倍增时间为 32h ,细胞的增殖能力随传代次数的增加而有所下降。结论 :密度梯度离心与贴壁培养相结合可以提高细胞分离效率。体外培养的兔骨髓间充质干细胞生长稳定 ,增殖力强 ,可以作为组织工程的种子细胞。

引用

页码：12 / 15

页数：4

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