实时荧光聚合酶链反应检测粪便标本中的金黄色葡萄球菌肠毒素A、D

被引：5

作者：

张红河 ^{[1
]}

范建中 ^{[1
]}

董晓勤 ^{[1
]}

张卫英 ^{[1
]}

王贤军 ^{[1
]}

陈瑜 ^{[2
]}

机构：

[1] 杭州市第一人民医院基因诊断实验室

[2] 浙江大学医学院附属第一医院

来源：

中国卫生检验杂志 | 2007年 / 02期

关键词：

金黄色葡萄球菌; 肠毒素; 聚合酶链反应;

D O I：

暂无

中图分类号：

R450 [];

学科分类号：

100215 ;

摘要：

目的:建立一种快速、准确、特异、定量检测金黄色葡萄球菌及其肠毒素A、D的方法并探讨肠毒素在食物中毒引起腹泻中的意义。方法:选择femB、SEA和SED基因分别作为金黄色葡萄球菌菌株、肠毒素A、肠毒素D的靶序列,设计合成引物和探针;收集食物中毒导致腹泻患者粪便标本487份,并对其进行检测分析。结果:采用荧光聚合酶链反应检测金黄色葡萄球菌和肠毒素A的灵敏度为1.0×102拷贝,而检测肠毒素D的灵敏度为1.0×103拷贝;487份粪便标本中检出金黄色葡萄球菌femB基因72例(14.8%),检出产肠毒素A菌株为11例(15.3%,11/72),产肠毒素D菌株为9例(12.5%,9/72),同时产肠毒素A、D菌株1例(1.4%,1/72)。结论:应用Taqm an探针实时荧光PCR技术能够快速、准确检测金黄色葡萄球菌及其肠毒素,适合于大样本筛查。

引用

页码：204 / 205+281 +281

页数：3

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