酒精处理影响青春期大鼠海马CA1区BDNF和TrkB的表达

被引:3
作者
王建中
刘丹
宋岭
崔涛涛
张建一
李昌琪
机构
[1] 中南大学湘雅医学院人体解剖学与神经生物学系
关键词
酒精; 海马; BDNF; TrkB; 青春期大鼠;
D O I
暂无
中图分类号
R361.2 [];
学科分类号
摘要
为探讨青春期饮酒致学习记忆力下降的可能机制,本研究观察了青春期大鼠酒精处理后海马CA1区脑源性神经营养因子(BDNF)和酪氨酸激酶受体B(TrkB)的表达变化。实验选用30d龄SD雄性大鼠,以25%的酒精按8g/kg/d灌胃,连续灌7d,动物分别在停酒后0d,3d,7d和14d处死;对照组以等量生理盐水代替酒精按同样方法处理。用免疫组织化学方法(ABC法)检测海马CA1区BDNF和TrkB的表达,Motic3.2图像分析系统测定免疫阳性产物的平均灰度值。结果显示,BDNF的表达在停酒后0d和14d,实验组与相应对照组相比差异无显著性(P>0.05);3d显著升高(P<0.05);7d明显下降(P<0.05)。TrkB的表达在停酒后0d,3d,7d,实验组与相应对照组相比差异无显著性(P>0.05);14d显著下降(P<0.05)。以上结果提示,BDNF表达的相对不足可能是青春期饮酒致学习记忆力持续性下降的原因之一。
引用
收藏
页码:11 / 15
页数:5
相关论文
共 9 条
  • [1] 酒精处理对青春期大鼠海马CA1区NGF和TrkA表达的影响
    王建中
    刘丹
    宋岭
    崔涛涛
    张建一
    李昌琪
    [J]. 神经解剖学杂志, 2008, (02) : 195 - 198
  • [2] BDNF regulates the expression and traffic of NMDA receptors in cultured hippocampal neurons
    Caldeira, Margarida V.
    Melo, Carlos V.
    Pereira, Daniela B.
    Carvalho, Ricardo F.
    Carvalho, Ana Luisa
    Duarte, Carlos B.
    [J]. MOLECULAR AND CELLULAR NEUROSCIENCE, 2007, 35 (02) : 208 - 219
  • [3] Chronic intermittent ethanol exposure during adolescence reduces the effect of ethanol challenge on hippocampal allopregnanolone levels and Morris water maze task performance
    Silvers, Janelle M.
    Tokunaga, Sayaka
    Mittleman, Guy
    O'Buckley, Todd
    Morrow, A. Leslie
    Matthews, Douglas B.
    [J]. ALCOHOL, 2006, 39 (03) : 151 - 158
  • [4] N-Methyl-D-aspartate subunit expression during mouse development altered by in utero alcohol exposure
    Toso, L
    Poggi, SH
    Abebe, D
    Roberson, R
    Dunlap, V
    Park, J
    Spong, CY
    [J]. AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2005, 193 (04) : 1534 - 1539
  • [5] Attenuation of brain derived neurotrophic factor (BDNF) by ethanol and cytoprotective effect of exogenous BDNF against ethanol damage in neuronal cells
    Sakai, R
    Ukai, W
    Sohma, H
    Hashimoto, E
    Yamamoto, M
    Ikeda, H
    Saito, T
    [J]. JOURNAL OF NEURAL TRANSMISSION, 2005, 112 (08) : 1005 - 1013
  • [6] Brain-derived neurotrophic factor acutely enhances tyrosine phosphorylation of the AMPA receptor subunit GluR1 via NMDA receptor-dependent mechanisms[J] . Kuo Wu,Guo-wei Len,Geoff McAuliffe,Chia Ma,Jessica P. Tai,Fei Xu,Ira B. Black.Molecular Brain Research . 2004 (1)
  • [7] Repeated episodic exposure to ethanol affects neurotrophin content in the forebrain of the mature rat
    Miller, MW
    [J]. EXPERIMENTAL NEUROLOGY, 2004, 189 (01) : 173 - 181
  • [8] BDNF acutely increases tyrosine phosphorylation of the NMDA receptor subunit 2B in cortical and hippocampal postsynaptic densities
    Lin, SY
    Wu, K
    Levine, ES
    Mount, HTJ
    Suen, PC
    Black, IB
    [J]. MOLECULAR BRAIN RESEARCH, 1998, 55 (01): : 20 - 27
  • [9] Developmental differences in the acquisition of tolerance to ethanol
    Swartzwelder, HS
    Richardson, RC
    Markwiese-Foerch, B
    Wilson, WA
    Little, PJ
    [J]. ALCOHOL, 1998, 15 (04) : 311 - 314