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一种分离葡萄糖氧化酶的方法
被引：1
作者：
戴国亮
郎爱东
李津如
江龙
机构：
[1] 中国科学院化学研究所分子科学中心!北京
来源：
关键词：
葡萄糖氧化酶;
分离;
活性;
构象;
D O I：
暂无
中图分类号：
O652.6 [富集方法、分离方法];
学科分类号：
070302 ;
081704 ;
摘要：
A method for separation of glucose oxidase(GOD) has been developed. A chroma tographic column is filled with an anion exchanger of styrene divinyl benzene matrix conta ining quaternary ammonium salt. The buffered phosphate and acetate solutions were used as eluents. Two GOD components(GOD A and GOD B) were separated by this method. Their enzyme activity and ζ potential values were 86 9, 27 3, and -4 3 mV, -6 4 mV, respecti vely . The simplicity and low cost of the method make it valuable for practical applications.
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页码：171 / 173
页数：3
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