运用表面等离子共振(SPR)生物传感器检测转基因玉米的研究

被引：9

作者：

肖守斌

机构：

[1] 湖南永州职业技术学院

来源：

玉米科学 | 2009年 / 02期

关键词：

表面等离子共振(SPR); DNA生物传感; 自组装单分子生物膜技术(SAM); 转基因玉米; Biacore XTM;

D O I：

10.13597/j.cnki.maize.science.2009.02.011

中图分类号：

S513 [玉米（玉蜀黍）];

学科分类号：

摘要：

采用自组装单分子生物膜技术(Self-assembled Monolayer,SAM),将带巯基头的35S启动子寡聚核苷酸探针修饰到镀金的Biacore芯片表面,建立了一种简单、快速、灵敏的表面等离子共振(surface Plasmon Resonance,SPR)DNA生物传感检测系统。研究结果表明,优化的表面等离子共振DNA传感器能够成功地用于转基因玉米PCR扩增样品的检测。

引用

页码：38 / 43

页数：6

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