炎症微环境下重组人釉原蛋白对人牙周膜成纤维细胞炎症因子表达的影响

被引：5

作者：

廖蔚文

宋忠臣

束蓉

董家辰

机构：

[1] 上海交通大学医学院附属第九人民医院牙周病科口腔医学院上海市口腔医学重点实验室

来源：

上海交通大学学报(医学版) | 2016年 / 36卷 / 01期

关键词：

牙周膜成纤维细胞; 炎症微环境; 重组人釉原蛋白; 炎症因子;

D O I：

暂无

中图分类号：

R781.4 [牙周病];

学科分类号：

摘要：

目的观察在炎症微环境下,重组人釉原蛋白(rhAm)对体外培养的人牙周膜成纤维细胞(hPDLCs)炎症因子表达的影响。方法体外培养hPDLCs,采用免疫组织化学染色法鉴定hPDLCs。用10μg/m L牙龈卟啉单胞菌(Porphyromonas gingivalis)脂多糖(LPS)模拟炎症微环境,选取20μg/m L rhAm作用于hPDLCs,运用实时荧光定量PCR和ELISA技术检测炎症因子白介素(IL)-1β、IL-6和IL-8的表达。结果免疫组织化学染色结果证实,培养细胞为hPDLCs。在所检测的4个时间点(6、12、24、72 h),10μg/m L P.gingivalis LPS均可诱导IL-1β、IL-6和IL-8的表达;加入20μg/mL rhAm后,各因子表达均有所降低。结论 rhAm可以抑制炎症微环境下h PDLCs炎症因子的表达。

引用

页码：33 / 37

页数：5

共 13 条

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