粉防已碱对人卵巢癌细胞株A2780增殖与凋亡的影响附视频

被引：10

作者：

韩晓兵

柳友清

卢运萍

马丁华

机构：

[1] 中科技大学同济医学院附属同济医院肿瘤生物医学中心

来源：

中国肿瘤临床 | 2006年 / 04期

关键词：

粉防已碱; 细胞周期; 凋亡; 卵巢癌;

D O I：

暂无

中图分类号：

R737.31 [卵巢肿瘤];

学科分类号：

摘要：

目的:通过观察粉防已碱对人卵巢癌A2780细胞增殖与凋亡的影响,初步探讨粉防已碱对卵巢癌的体外抗肿瘤效应。方法:采用MTT试验观察粉防已碱对A2780细胞的增殖抑制效应,利用细胞DNA琼脂糖凝胶电泳检测粉防已碱诱导肿瘤细胞凋亡的作用,通过流式细胞术观察粉防已碱对人卵巢癌A2780细胞周期的影响以及诱导凋亡的作用,以免疫印迹检测粉防已碱对p53、p21及Bax表达水平的影响。结果:MTT试验显示粉防已碱对人卵巢癌A2780细胞增殖有抑制作用,其抑制效应具有时间及浓度依赖的特点,琼脂糖凝胶电泳及流式细胞术表明粉防已碱可诱导A2780细胞凋亡,并引起G0/G1期阻滞,免疫印迹检测显示粉防已碱上调p53、p21及Bax蛋白表达。结论:粉防已碱对人卵巢癌A2780细胞增殖的抑制效应具有时间及浓度依赖性,并可诱导细胞凋亡,其抗肿瘤效应可能与上调p53、p21及Bax蛋白表达有关。

引用

页码：190 / 193

页数：4

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