硫和pH对蛋白核小球藻光照产氢的影响

被引:16
作者
贺立静 [1 ]
雷腊梅 [1 ]
龙敏南 [2 ]
韩博平 [1 ]
机构
[1] 暨南大学水生生物研究所
[2] 厦门大学生命科学学院
关键词
蛋白核小球藻; 产氢; pH; 硫;
D O I
暂无
中图分类号
Q945 [植物生理学];
学科分类号
071001 [植物学];
摘要
在有无硫及pH5.0-8.0下对蛋白核小球藻(Chlorella pyrenoidosa)光照产氢的影响进行了研究。结果表明,在持续光照(165μmolm-2s-1)条件下,从有硫培养液(TAP培养液)内叶绿素a含量、Fv/Fm值及ΦPSII值的变化表明蛋白核小球藻在pH6.0-7.0时生长最佳,生长旺盛易形成暂时的无氧环境而利于藻产氢。最高的产氢速率和总产氢量出现在pH7.0,分别是0.10mlmg-1chlh-1和1.39ml。从无硫培养液(TAP-S培养液)内叶绿素a含量、Fv/Fm值及ΦPSII值的变化表明蛋白核小球藻生长明显受抑制,形成的无氧环境持久,故产氢持久,总产氢量比有硫培养液内高。蛋白核小球藻在pH5.5培养液内的Fv/Fm值后期高于其他4种pH值的,表明潜在的PSII光化学效率高,在光照条件下产氢电子主要来源于PSII,故pH5.5的无硫培养液内藻的产氢速率和总产氢量最大,分别是0.58mlmg-1chlh-1和10.98ml。说明pH为5.5的无硫培养液是蛋白核小球藻产氢的最佳条件。
引用
收藏
页码:487 / 492
页数:6
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