骨髓基质细胞复合三维支架修复犬下颌骨节段性缺损

被引：1

作者：

何悦

张志愿

卢建熙

张秀丽

张陈平

机构：

[1] 上海交通大学医学院附属第九人民医院·口腔医学院口腔颌面外科上海市口腔医学研究所,上海市口腔医学重点实验室

来源：

中国口腔颌面外科杂志 | 2005年 / 04期

关键词：

骨髓基质细胞; β-磷酸三钙; 下颌骨; 节段性缺损; 犬;

D O I：

暂无

中图分类号：

R782 [口腔颌面部外科学];

学科分类号：

100302 ;

摘要：

目的:探讨犬骨髓基质细胞(bone marrow stromal cells,BMSCs)体外复合预制三维下颌骨缺损仿真β-磷酸三钙(β-TCP)支架对犬下颌骨节段性缺损进行修复的可行性。方法:通过计算机辅助设计/制作和快速原型技术,制作3cm长犬下颌骨节段性缺损β-磷酸三钙支架,体外接种第3代骨髓基质细胞,培养5d天后回植,对犬一侧下颌骨节段性缺损进行修复,并设单纯材料作为对照组。术后1周、1个月、3个月观察局部伤口情况,术后1、3个月行X线片和三维CT成像,术后3个月将标本进行生物力学检测和组织学观察。结果:X线片观察显示,术后3个月,对照组缺损区低密度影,BMSCs+β-TCP组缺损区见高密度组织;三维CT成像显示术后3个月,对照组修复区吸收明显,达1/2以上;BMSCs+β-TCP组修复区未见明显吸收;生物力学检测显示,对照组和BMSCs+β-TCP组的牙槽嵴高度分别为9.16mm和18.54mm,最大弯曲载荷分别为42.90N和102.77N,应力分别为1.930N/mm2和3.504N/mm2,应变分别为54.50%和16.98%。经统计学处理,均有显著性差异;组织学观察显示术后3个月,BMSCs+β-TCP组成骨良好,支架周围可见多核巨细胞吞噬材料,并可见软骨形成区。结论:应用β-TCP三维支架复合犬BMSCs进行犬下颌骨节段性缺损修复存在一定的可行性。

引用

页码：345 / 350

页数：6

共 12 条

[1]

Vascularized free bone grafts for maxillary and mandibular reconstruction. Misiek DJ. Atlas of the Oral and Maxillofacial Surgery Clinics of North America . 1994

[2]

Reconstruction of mandibular discontinuity defects with delayed nonvascularized free iliac crest bone grafts and endosseous implants:a clinical report. Hotz G. The Journal of ProsThetic Dentistry . 1996

[3]

Vascularized free fibular flap for mandibular reconstruction:a report of26cases. Munoz Guerra MF,Gias LN,et al. Journal of Oral and Maxillofacial Surgery . 2001

[4]

Mandibular osteomyelitis and fracture successfully treated with vascularised iliac bone graft in a patient with pycnodysostosis. Kato H,Matsuoka K,Kato N,et al. British Journal of Plastic Surgery . 2005

[5]

Osteogenic imprinting upstream of marrow stromal cell differentiation. Satomura K,Krebsbach P,Bianco P,et al. Journal of Cellular Biochemistry . 2000

[6]

Free composite myo-osseous flap with serratus anterior and rib:indications in head and neck reconstruction. Netscher D,Alford EL,Wigoda P,et al. Head and Neck . 1998

[7]

A comparison of vascularized and nonvascularized bone grafts for reconstruction of mandibular continuity defects. Pogrel MA,Podlesh S,Anthony JP,et al. Journal of Oral and Maxillofacial Surgery . 1997

[8]

Bone graft in the shape of human mandibular condyle reconstruction via seeding marrow-derived osteoblasts into coral in a nude mice model. Chen F,Mao T,Tao K,et al. Journal of Oral and Maxillofacial Surgery . 2002

[9]

Reconstruction of mandibular defects with autologous tissue-engineered bone. Abukawa H,Shin M,Williams WB,et al. Journal of Oral and Maxillofacial Surgery . 2004

[10]

Reconstruction in the head and neck regions with free radial forearm flaps and split-rib bone grafts. Lind MG,Arnander C,Gylbert L,et al. The American Journal of Surgery . 1987

← 1 2 →