阻断转化生长因子—β1信号传导治疗大鼠实验性肝纤维化

被引：20

作者：

徐新保

何振平

梁志清

冷希圣

机构：

[1] 北京大学人民医院肝胆外科中心,重庆第三军医大学西南肝胆外科医院,重庆第三军医大学西南医院妇产科,北京大学人民医院肝胆外科中心,

来源：

中华肝脏病杂志 | 2004年 / 05期

关键词：

肝纤维化; 转化生长因子-β; 腺病毒; 人; Smad4; 反义基因;

D O I：

暂无

中图分类号：

R575.2 [肝硬变];

学科分类号：

摘要：

目的观察腺病毒介导的反义Smad4基因阻断转化生长因子—β1（TGF—β1）信号传导后对肝纤维化的治疗作用。方法将腺病毒介导的反义Smad4基因导入四氯化碳/乙醇诱导的大鼠纤维化肝脏,用RT-PCR及western方法观察Smad4的表达,并观察肝脏的病理变化和胶原表达。结果纤维化肝脏中Smad4的表达较正常肝脏明显增强,Ⅰ型胶原增多。经转基因处理后大鼠纤维化肝脏中Smad4的表达明显弱于未治疗的纤维化肝脏,Ⅰ型胶原减少;且治疗组肝脏纤维间隔也明显减少,纤维化程度明显减轻。结论腺病毒介导的反义Smad4基因能有效阻断TGF—β信号传导系统,减少细胞外基质的产生,从而减轻肝纤维化的程度。

引用

页码：12 / 15

页数：4

共 9 条

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