cDNA微阵列技术研究干旱胁迫下星星草基因的表达

被引：4

作者：

梁也男

王玉成

刘桂丰

褚延广

机构：

[1] 东北林业大学林木遗传育种实验室

来源：

植物生理学通讯 | 2007年 / 05期

关键词：

DNA微阵列; 星星草; 干旱胁迫; 基因表达;

D O I：

10.13592/j.cnki.ppj.2007.05.027

中图分类号：

Q943.2 [植物基因工程]; S54 [饲料作物、牧草];

学科分类号：

071007 ; 090102 ; 090503 ; 0909 ;

摘要：

运用cDNA微阵列技术研究干旱胁迫下星星草基因的表达。制备了载有660条星星草单一基因的cDNA微阵列。分别对干旱胁迫和对照星星草的mRNA进行荧光标记,并与载有星星草基因的cDNA微阵列进行杂交,通过芯片的杂交信号强度分析,共获得22个下调表达和17个上调表达的基因。BLASTX分析表明这些基因按功能可以分为脱水保护、信号转导与调控、活性氧清除、代谢、核糖体蛋白等几大类。发现了一些与干旱胁迫相关的功能未知基因和新基因。

引用

页码：831 / 836

页数：6

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