基于16S rDNA高通量测序方法比较新疆西北部地区乳品中微生物的多样性

被引：55

作者：

张敏

张艳

黄丽丽

刘忆冬

周红

倪永清

机构：

[1] 石河子大学食品学院

来源：

食品科学 | 2017年 / 38卷 / 20期

关键词：

乳品; 微生物多样性; 高通量测序技术;

D O I：

暂无

中图分类号：

TS252.1 [基础科学];

学科分类号：

083203 ;

摘要：

为更加全面地了解新疆特色乳品中微生物多样性,比较不同动物来源的原奶和酸奶的细菌群落结构,运用高通量测序技术,对乳品中细菌16S r DNA V4-V5区测序,进而对新疆克州和塔城地区牛奶、驼奶、马奶、羊奶、酸牛奶、酸驼奶和酸马奶7种乳品中细菌群落组成和多样性进行分析。研究共获得539 557条有效序列,379个OTU。多样性分析表明,原奶样品中细菌Shannon-Wiener指数明显高于酸奶样品。微生物群落组成分析发现,不同乳品之间菌群组成差异较大。7种乳品中的菌群均以厚壁菌门和变形菌门为主,但原奶样品主要以变形菌门为主,而酸奶样品主要以厚壁菌门为主。在属水平上,牛奶主要以假单胞菌属(Pseudomonas)为主,驼奶主要以埃希菌属-志贺菌属(Escherichia-Shigella),马奶主要以明串珠菌属(Leuconostoc)为主,羊奶中的优势菌属为乳球菌属(Lactococcus),而酸牛奶、酸驼奶和酸马奶都是以乳杆菌属(Lactobacillus)为优势菌属。不同动物来源的原奶和酸奶样品中的微生物多样性存在显著差异,并且原奶中检测到的环境污染菌和致病菌(或条件致病菌)的丰度也相对较高。本研究结果将为准确评估乳品中的微生物群落对新疆地区少数民族健康的影响提供一定的数据基础。

引用

页码：27 / 33

页数：7

共 18 条

[1]

From field to fermentation: The origins of Lactococcus lactis and its domestication to the dairy environment[J] . Daniel Cavanagh,Gerald F. Fitzgerald,Olivia McAuliffe.Food Microbiology . 2015

[2] The complex microbiota of raw milk [J].

Quigley, Lisa ;

O'Sullivan, Orla ;

Stanton, Catherine ;

Beresford, Tom P. ;

Ross, R. Paul ;

Fitzgerald, Gerald F. ;

Cotter, Paul D. .

FEMS MICROBIOLOGY REVIEWS, 2013, 37 (05) :664-698

[3] Assessment of the microbial diversity of Brazilian kefir grains by PCR-DGGE and pyrosequencing analysis [J].

Leite, A. M. O. ;

Mayo, B. ;

Rachid, C. T. C. C. ;

Peixoto, R. S. ;

Silva, J. T. ;

Paschoalin, V. M. F. ;

Delgado, S. .

FOOD MICROBIOLOGY, 2012, 31 (02) :215-221

[4]

Characterization of bacterial populations in Danish raw milk cheeses made with different starter cultures by denaturating gradient gel electrophoresis and pyrosequencing[J] . International Dairy Journal . 2010 (3)

[5] Terminal-restriction fragment length polymorphism and automated ribosomal intergenic spacer analysis profiling of fungal communities in Camembert cheese [J].

Arteau, Marianne ;

Labrie, Steve ;

Roy, Denis .

INTERNATIONAL DAIRY JOURNAL, 2010, 20 (08) :545-554

[6] Culture-independent methods for identifying microbial communities in cheese [J].

Jany, Jean-Luc ;

Barbier, Georges .

FOOD MICROBIOLOGY, 2008, 25 (07) :839-848

[7] Microbiological study of lactic acid bacteria in kefir grains by culture-dependent and culture-independent methods [J].

Chen, Hsi-Chia ;

Wang, Sheng-Yao ;

Chen, Ming-Ju .

FOOD MICROBIOLOGY, 2008, 25 (03) :492-501

[8]

Microbiological characterisation of Robiola di Roccaverano cheese using PCR–DGGE[J] . Silvia Bonetta,Sara Bonetta,Elisabetta Carraro,Kalliopi Rantsiou,Luca Cocolin.Food Microbiology . 2008 (6)

[9]

Analysis of bacterial community during the fermentation of pulque, a traditional Mexican alcoholic beverage, using a polyphasic approach[J] . Adelfo Escalante,Martha Giles-Gómez,Georgina Hernández,María Soledad Córdova-Aguilar,Agustín López-Munguía,Guillermo Gosset,Francisco Bolívar.International Journal of Food Microbiology . 2008 (2)

[10]

Diversity of lactic acid bacteria and yeasts in Airag and Tarag, traditional fermented milk products of Mongolia[J] . Koichi Watanabe,Junji Fujimoto,Masae Sasamoto,Jamyan Dugersuren,Tseveendori Tumursuh,Shirchin Demberel.World Journal of Microbiology and Biotechnology . 2008 (8)

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