急性缺血诱导大鼠心肌细胞凋亡的时间演变和空间分布

被引：9

作者：

刘宗军

江智文

温沁竹

机构：

[1] 上海市第一人民医院心血管内科!上海,上海市第一人民医院心血管内科!上海,上海市第一人民医院心血管内科!上海

来源：

中国病理生理杂志 | 2000年 / 11期

关键词：

心肌; 细胞; 局部缺血; 凋亡;

D O I：

暂无

中图分类号：

R363.1 [病因学];

学科分类号：

100104 ;

摘要：

目的 :探讨急性缺血诱导大鼠心肌细胞凋亡的时间演变和空间分布规律。方法 :以DNA电泳和TUNEL分析检测SD大鼠心肌的细胞凋亡情况。结果 :缺血 2h开始出现DNA梯形条带和TUNEL阳性细胞 ,DNA条带和凋亡指数随缺血时间延长而显著增加 (P <0 0 1)。TUNEL阳性心肌细胞在缺血区散在分布 ,缺血边缘区凋亡指数显著高于缺血中央区 (P <0 0 5 ) ,缺血区内膜下凋亡指数显著高于外膜下 (P <0 0 1)。结论 :在本实验设定的时间范围内 ,急性缺血诱导的心肌细胞凋亡与缺血时间具有时效关系 ;调亡的心肌细胞在缺血区散在分布 ,以缺血区两侧边缘和内膜下心肌多见。

引用

页码：70 / 73+100

页数：5

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