INHIBITORY EFFECT OF BOSCHNIAKIA ROSSICA ON DEN-INDUCED PRECANCEROUS HEPATIC FOCI AND ITS ANTIOXIDATIVE ACTIVITIES IN RATS

Objective: To investigate the inhibitory effect ofBoschniakia rossica(BR) on rat precancerous hepatic fociinduced by diethylnitrosamine (DEN) and its antioxidativeaedvities. Methods: The expression of tumor markerPlacental form glutathione S-transferase (GST-P), p53 andp21 protein were investigated by immunohistochendstrytechniques using ABC method. TNF-α was measured byELISA and antioxidative activities of SOD, MDA, GSH-Px,GST and CAT were investigated by colorimetric method inrat serum and mitochondria of liver cells. Results: The500mg/kg of BR-H2O extract fraction from BR-methanolextract had inhibitory effect on the formation of DENinduced GST-P-positive foci in rat liver and the expressionof mutant p53 and p21 protein was lower than that ofhepatic precancerous lesions. The serum TNF-α wasincreased by the administration of BR extract in the earlystage of chemical hepatocarcinogenesis in rat livers. Thesertun and liver cells mitochondria activities of SOD andGSH-Px rose again in rats administered with BR-H2Oextract and the increasing activity 0f GST and content ofMDA in the hepatic precancerous were decreased by theBR-H2O extract. Conclusion: These results indicated thatBR-H2O extract has inhibitory effect on DEN-inducedprecancerous hepatic foci in rats and induced TNF-αproduction in rats. The antioxidative action was exhibitedby the administration of BR-H2O extract in the early stageof chemical bepatocarcinogenesis in rat livers.