FAK反义寡核苷酸促进人肺动脉平滑肌细胞凋亡

被引：13

作者：

林春龙

张珍祥

机构：

[1] 华中科技大学同济医学院附属同济医院呼吸内科

来源：

中国病理生理杂志 | 2006年 / 02期

关键词：

粘着斑激酶; 肺动脉; 细胞凋亡; 半胱氨酸天冬氨酸蛋白酶3;

D O I：

暂无

中图分类号：

R363 [病理生理学];

学科分类号：

100104 ;

摘要：

目的:了解黏着斑激酶(focal adhesion kinase FAK)是否参与人肺动脉平滑肌细胞(HPASMCs)凋亡。方法:纤黏连蛋白(FN40mg/L)刺激下培养的HPASMCs被用来进行反义寡核苷酸(antisense oligonucleotides,ASODNs)转染以做进一步实验。采用免疫印迹方法测定FAK蛋白质及凋亡蛋白半光氨酸蛋白酶-3(caspase-3)的表达,TUNEL法检测细胞凋亡。结果:免疫印迹方法测定结果表明,应用FAKASODNs转染后HPASMCs中FAK的表达明显低于转染前(P<0·01);caspase-3在应用FAKASODNs转染后高于转染前(P<0·05),TUNEL法检测结果表明FAKASODNs转染后细胞凋亡增加。结论:本实验结果表明FAKASODNs抑制细胞增生,促进细胞凋亡。其机制很可能是经caspase-3信号通路促进细胞凋亡。

引用

页码：288 / 291

页数：4

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