基于叠氮溴化丙锭与恒温核酸扩增技术快速检测亚致死状态食源金黄色葡萄球菌

被引：7

作者：

曹潇 ^{[1
]}

赵力超 ^{[1
,2
]}

杨翠琪 ^{[1
]}

谷立慧 ^{[1
]}

欧阳广宇 ^{[1
]}

陈金 ^{[1
]}

陈思锴 ^{[1
]}

谭绮雯 ^{[1
]}

王丽 ^{[1
,2
]}

机构：

[1] 华南农业大学食品学院

[2] 广东省天然产物绿色加工与产品安全重点实验室

来源：

食品科学 | 2016年 / 37卷 / 24期

基金：

广东省科技计划;

关键词：

食品安全; 金黄色葡萄球菌; 亚致死状态; 叠氮溴化丙锭-环介导等温扩增技术; 快速检测;

D O I：

暂无

中图分类号：

TS207.4 [食品的微生物检验];

学科分类号：

083201 ;

摘要：

建立一种将荧光染料叠氮溴化丙锭(propidium monoazide,PMA)与环介导等温扩增(loop-mediated isothermal amplification,LAMP)技术相结合的方法,用于快速高效检测金黄色葡萄球菌(Staphylococcus aureus)。同时,采用人工污染金黄色葡萄球菌的速冻水饺和奶粉作为食品样品,研究PMA-LAMP方法的检测灵敏度。结果表明,PMA溶液质量浓度3μg/mL,650 W卤素灯下曝光5 min,PMA能够完全抑制1.2×10～7 copies/mL金黄色葡萄球菌死菌核酸扩增。PMA-LAMP方法能够在恒温65℃、60 min内完成对亚致死型金黄色葡萄球菌特异性nuc基因的特异性检测,其对亚致死状态金黄色葡萄球菌的检出限为34 CFU/mL,对食物样品速冻水饺和奶粉的检出限分别为17 CFU/m L和1.70×10～2 CFU/mL。建立的PMA-LAMP方法可以有效检测亚致死态金黄色葡萄球菌,提供了一种新的检测技术和解决方案。

引用

页码：149 / 155

页数：7

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