淹水玉米幼苗根尖分生细胞内Ca2+超微细胞化学定位

被引:3
作者
魏和平
王建波
利容千
吴甘霖
机构
[1] 安庆师范学院生命科学系
[2] 武汉大学生命科学院
[3] 安庆师范学院生命科学系 安徽安庆
[4] 武汉
[5] 安徽安庆
关键词
淹水; 玉米; 根尖分生细胞; Ca2+定位;
D O I
暂无
中图分类号
S513 [玉米(玉蜀黍)];
学科分类号
0901 ;
摘要
采用焦锑酸钾沉淀法,对遭受淹水胁迫的玉米幼苗初生根根尖分生细胞内钙离子分布变化情况进行了电镜细胞化学观察。在正常状态下,根尖分生细胞内Ca2+沉淀颗粒的分布较少,主要位于细胞核和细胞质中。在淹水1h后,根尖分生细胞内呈现有大量Ca2+沉淀颗粒分布,细胞核和细胞质中分布的Ca2+沉淀颗粒密度,远大于正常细胞。随着淹水时间的延长,根尖分生细胞的细胞核和细胞质中分布的Ca2+沉淀颗粒呈现不断增多的趋势,而液泡中分布的Ca2+沉淀颗粒则逐步明显减少。根据实验结果本文对受淹根尖分生细胞的死亡与Ca2+分布变化的关系进行了研究。
引用
收藏
页码:20 / 23
页数:4
相关论文
共 19 条
[1]   淹涝胁迫对水稻生长发育危害与耐淹性机理研究的进展 [J].
李玉昌 ;
李阳生 ;
李绍清 .
中国水稻科学, 1998, (S1) :70-76
[2]  
Root respiration and carbohydrates status two wheat genotypes in responses to hypoxia and ethylene. Huang B,Jackson JW. Crop Science . 1995
[3]  
Calcium signaling. David EC. Cell . 1995
[4]  
Effect of heat stress on calcium ultrastructural distribution in pepper anther. Yan CHL,Wang JB,Li RQ. Environmental and Experimental Botany . 2002
[5]  
Elevation of cytosolic calcium precedes anoxic gene expression in maize suspension-cultured cells. Subbaiah CC,Bush DS,Sachs MM. The Plant Cell . 1994
[6]  
Investigation of the calcium transporting ATPase at the endoplasmic reticulum and plasma membrane of red beet(Beta vulgaris). Thomson LJ,Xing T,Hall JL et al. Plant Physiology . 1993
[7]  
Enhancement of submergence tolerance in transgemc rice overproducing pyruvate decarboxylase. Quimio CA,Torrizo LB,Setter TL et al. Journal of Plant Physiology .
[8]  
Enhanced low oxygen survival in Arabidopsis through increased metabolic flux in the fermentative pathway. Ismond KP,Dolferus R,Pauw MD et al. Plant Physiology . 2003
[9]  
Calcium regulation iplant cells and its role in signaling. Bush DS. Annual Review of Plant Physiology . 1995
[10]  
Cytochemical localization of calcium and Ca2+ -ATPase activity in plant cells under chilling-sensative maize and chilling-insensative winter wheat. Jian LC,Li JH,Chen WP et al. Plant and Cell Physiology . 1999