超高效亲水色谱-串联质谱法快速检测鸡肉及其制品中的利巴韦林及其代谢物的总残留量

被引：21

作者：

祝伟霞 ^{[1
]}

杨冀州 ^{[1
]}

袁萍 ^{[1
]}

孙转莲 ^{[1
]}

王彩娟 ^{[2
]}

孙武勇 ^{[1
]}

张书胜 ^{[2
]}

机构：

[1] 河南出入境检验检疫局

[2] 郑州大学化学与分子工程学院

来源：

色谱 | 2013年 / 10期

关键词：

液相色谱-串联质谱; 亲水色谱; 分散固相萃取; 利巴韦林; 速成鸡; 鸡肉制品;

D O I：

暂无

中图分类号：

O657.63 [质谱分析]; TS251.7 [产品标准与检验];

学科分类号：

070302 ; 081704 ; 083203 ;

摘要：

建立了鸡肉及其制品中利巴韦林和磷酸化利巴韦林代谢物总残留量的亲水作用色谱-串联质谱分析方法（HILIC-MS/MS）。样品采用含1%（v/v）乙酸的乙腈超声提取,于37℃条件下以酸性磷酸酶酶解,正己烷除脂后用C18与PSA填料分散固相萃取净化。采用酰胺基超高效亲水作用色谱柱,在乙腈和0.1%甲酸组成的梯度洗脱流动相中分离,在电喷雾正离子电离多反应监测模式下监测,结果表明,样品中利巴韦林残留量在1～200μg/kg之间时,线性相关系数大于0.999,方法检出限（LOD,S/N≥3）为1μg/kg,定量限（LOQ,S/N≥10）为5μg/kg,在3个添加水平下的回收率为67.8%112.7%,相对标准偏差为6.1%13.6%。经测定多种实际样品,证明该方法简便、快速、准确,可以满足日常鸡肉及其制品中利巴韦林及其代谢物总残留量的分析要求。

引用

页码：934 / 938

页数：5

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