氨甲喋呤致口腔粘膜损伤动物模型的建立

AIM:To explore the model of methotrexate(MTX)-induced mic e oral mucosa lesions METHOD:A total of 282 mice were randomly d ivided into seven groups as follows:control,MTX10,20,30,40,50,75 mg/ml The physiological saline solution (05 ml) was ip injecte d into mice in control group The dose of MTX (05 ml) was ip injected int o mic e in other groups only once During 10 consecutive days after administration of MTX,3 mice were sacrificed in each day in all the groups and the patholog ical chang es of oral mucosa were examed under the optical microscope RE SULT: The incidence(45%～63%) and the integral rate(194%～56%) of mice ora l mu cosa lesions in group of intermediate-dose MTX (IDMTX) (20～40 mg/kg) were sign ifi cantly higher than those in other groups The death rate (not more than 5%) in group of IDMTX was lower than that in other groups CONCLUSION:As a result of induction of higher lesion rate and lower death rate of mice oral mucosa by IDMTX (2 0～40 mg/kg),the model of IDMTX-induced oral mucosa lesion could be applie d into clinical or basic studies