改造中国仓鼠卵巢细胞

被引：8

作者：

来大志

齐连权

于长明

王海涛

陈薇

机构：

[1] 北京微生物流行病研究所

[2] 北京微生物流行病研究所北京

[3] 北京

来源：

生物工程学报 | 2002年 / 04期

关键词：

CHO细胞; 改造; 大规模培养; 细胞凋亡; 细胞黏附; 细胞周期;

D O I：

10.13345/j.cjb.2002.04.004

中图分类号：

Q813 [细胞工程];

学科分类号：

0836 ; 090102 ;

摘要：

与原核细胞、酵母细胞以及昆虫细胞相比 ,中国仓鼠卵巢细胞 (CHO)作为宿主细胞表达的外源蛋白最接近其天然构象 ,因而CHO细胞表达系统是生物工程制药最为理想的表达系统。但这种系统也存在诸多缺点。如在大规模培养中CHO细胞会面临着对无血清培养基的适应性差、细胞无限度增殖以及细胞凋亡等很多难题。所以除了在培养基、培养条件和表达载体方面下功夫优化该系统外 ,对CHO细胞本身进行改造已成为优化CHO表达系统的另一热点。

引用

页码：415 / 419

页数：5

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