空肠弯曲菌的磁捕获-荧光PCR检测方法的建立

被引：18

作者：

刘光明

苏文金

蔡慧农

谢明星

刘棠

彭小莉

机构：

[1] 厦门出入境检验检疫局,集美大学生物工程学院,集美大学生物工程学院,厦门出入境检验检疫局,厦门出入境检验检疫局,厦门出入境检验检疫局厦门汕头大学生物学系,汕头,厦门,厦门,厦门,厦门,厦门

来源：

生物工程学报 | 2005年 / 02期

关键词：

空肠弯曲菌; 免疫磁珠捕获; 荧光PCR; 检测;

D O I：

10.13345/j.cjb.2005.02.034

中图分类号：

R446.5 [微生物学检验];

学科分类号：

摘要：

为提高畜禽类食品中空肠弯曲菌的检出率和灵敏度 ,应用抗血清和磁性微珠首次制备弯曲菌免疫磁珠 ,利用弯曲菌免疫磁珠直接捕获检样中目的菌 ,不需要增菌培养 ;通过荧光PCR检测鞭毛蛋白A（flaA）基因和或马尿酸酶（hipO）基因 ,首次建立空肠弯曲菌的磁捕获_荧光聚合酶链反应（IMCFPCR）方法。IMCFPCR法检测空肠弯曲菌方法简便易行 ,可在 2 4h内完成 ,特异性好 ,检测低限达到 10cfu mL ,抗干扰性强。IMCFPCR方法可望解决非可培养状态的空肠弯曲菌检测难题 ,是一种适用于检验检疫、卫生防疫和农产品安全检验等领域的快速方法。

引用

页码：336 / 340

页数：5

共 9 条

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