两种血培养瓶在含抗菌药物血样中检测细菌的性能比较

被引：9

作者：

高晓东

胡必杰

周春妹

谢红梅

黄声雷

夏文松

机构：

[1] 复旦大学附属中山医院微生物实验室

来源：

中华医院感染学杂志 | 2009年 / 19卷 / 06期

关键词：

血培养; 抗菌药物; 树脂瓶; 活性炭瓶; 检出时间;

D O I：

暂无

中图分类号：

R446.5 [微生物学检验];

学科分类号：

100208 ;

摘要：

目的比较BD公司BACTEC PLUS+血培养瓶(BD-P瓶)与法国生物梅里埃公司BacT/Alert FA血培养瓶(下简称Bio-F瓶)对含抗菌药物血样中细菌的检测性能。方法将含菌液、抗菌药物、无菌血共5 ml分别注入BD公司BACTEC Standard 10标准瓶(下简称BD-S瓶)、BD-P树脂瓶、法国生物梅里埃公司BacT/Alert SA标准瓶(下简称Bio-S瓶)、Bio-F活性炭瓶的4种血培养瓶内,放入自动化仪器内进行培养,然后记录5 d内阳性瓶的检出时间,实验结束后对4种不同血培养瓶内的检出率及检出时间进行比较。结果含抗菌药物的150瓶中普通瓶仅54瓶被检出,而有吸附能力的150瓶仅32瓶未被检出;BD-P瓶和Bio-F瓶对阿米卡星、氟康唑均有很好的吸附能力,检出时间二者差异无统计学意义,均P>0.05;对替考拉宁Bio-F血培养瓶的检出时间短于BD-P血培养瓶,P=0.009,高浓度的阿米卡星仅Bio-F血培养瓶才检出;而BD-P瓶对于环丙沙星、β-内酰胺类抗菌药物、万古霉素的检出时间明显要短于Bio-F,P值分别为<0.001、0.023和0.009。结论具有抗菌药物吸附能力的培养瓶的检出率要明显高于普通血培养瓶,检出速度明显提高;BD的树脂瓶对绝大多数抗菌药物的吸附能力优于生物梅里埃的活性炭瓶。

引用

页码：708 / 712

页数：5

共 10 条

[1] 活性炭体外对万古霉素等7种抗生素吸附的实验研究 [J].

赵旺胜 ;

唐新 ;

童明庆 .

中华医院感染学杂志, 2002, (01) :21-22+31

[2]

Central venous catheters:can we control the in-fections. Leitman MI. Current Surgery . 1999

[3]

The attributable cost,and lengthof hospital stay of central line associated blood stream infectionin intensive care units in Brazil.A prospective,matched analy-sis. Salomao R,Rosenthal VD. American Journal of Infection Control . 2006

[4]

Laboratory diagnosis of bacteremia and fungemia. Magadia RR,Weinstein MP. Infectious Diseases in Clinical Practice . 2001

[5]

Controlled clinical evaluationof BACTEC Plus Aerobic/F and BacT/Alert aerobic FAN bottles fordetection of bloodstream infections. Pohlman JK,,Kirkley BA,Easley KA. Journal of Clinical Microbiology . 1995

[6]

Comparison of the effect of delayed entryinto 2 different blood culture systems(BACTEC 9240 andBacT/Alert 3D)on culture positivity. Akan QA,Yildiz E. Diagn Microbiol In-fect Dis . 2006

[7]

Use of simulated blood cultures for an-tibiotic effect on time to detection of the two blood culture sys-tems BacT/Alert and BACTEC 9240. Vigano EF,Vasconi E. New Microbiologica . 2004

[8]

Extra mortality of nosocomialinfections in adult ICUs of 9 hospitals of Argentina.Findingsof the International Nosocomial Infection Control Consortium(INICC). Rosenthal VD,Sztokhamer D. American Journal of Infection Control . 2006

[9]

The clinical significance of positive blood cultures in the 1990s: a prospective comprehensive evaluation of microbiology, epidemiology and outcome of bacteremia and fungemia in adults. W einstein,MP,Towns,ML,Quartey,SM,Mirrett,S,Reimer,LG,Parmigiani,G,Reller,LB. Clinical Infectious Diseases . 1997

[10]

Comparative study of BacT/Alert FAN bottles and standard BacT/Alert bottles. Gibb,AP,Hill,B,Chorel,B. Diagnostic Microbiology and Infectious Disease . 1998

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