新型抗风湿药艾拉莫德抑制Th17细胞分化的调节作用

被引：8

作者：

孙晓萱

甘晓霞

谈文峰

魏禺

华敏慧

张缪佳

机构：

[1] 南京医科大学第一附属医院风湿免疫科

来源：

现代免疫学 | 2013年 / 33卷 / 05期

关键词：

Th17细胞; 类风湿关节炎; 艾拉莫德;

D O I：

暂无

中图分类号：

R392 [医学免疫学];

学科分类号：

100108 [医学免疫学];

摘要：

通过研究艾拉莫德抑制Th17细胞分化的调节作用,为临床治疗Th17相关性炎症与自身免疫病提供理论与实验依据。取C57BL/6小鼠脾脏淋巴细胞,磁珠分选CD4+CD62L+T细胞(nave T细胞),以anti-CD3、anti-CD28活化T细胞,同时加入IL-6、TGF-β及IL-23诱导nave T细胞向Th17细胞分化。在上述培养体系中加入艾拉莫德(53.4mmol/L)刺激细胞,并以空白组和MTX(0.3mmol/L)组作为对照。培养3d后收集细胞,流式细胞仪检测CD4+IL-17+Th17细胞的表达;酶联免疫吸附测定(Enzyme linked immunosorbent assay,ELISA)检测细胞培养上清白细胞介素-17(Interleukin-17,IL-17)水平;实时荧光定量PCR(real-time PCR)检测细胞IL-17和视黄酸相关的孤儿受体-γt(ROR-γt)基因表达水平。结果提示:在细胞水平,艾拉莫德对Th17细胞的抑制作用强于MTX;在蛋白水平,艾拉莫德对IL-17的抑制作用明显强于MTX(P<0.05);在基因水平,与MTX相比,艾拉莫德对ROR-γt mRNA和IL-17mRNA表达的抑制作用更强(P<0.05)。结果说明,艾拉莫德对Th17细胞的抑制作用强于MTX,艾拉莫德在控制RA病情中的作用可能优于MTX。

引用

页码：365 / 369

页数：5

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