MRTF-A通过NF-κB参与AGEs诱导肾小球系膜细胞中FN、ICAM-1的表达

被引：11

作者：

陈秋红 ^{[1
]}

陈志泉 ^{[1
]}

黄佳妮 ^{[1
]}

黄河清 ^{[1
,2
]}

机构：

[1] 中山大学药学院

[2] 广东省疾病模式动物工程技术研究中心

来源：

中国药理学通报 | 2017年 / 04期

基金：

广东省科技计划;

关键词：

糖尿病肾病; 肾小球系膜细胞; AGEs; MRTF-A; p65; FN; ICAM-1;

D O I：

暂无

中图分类号：

R587.2 [糖尿病性昏迷及其他并发症]; R692.9 [其他疾病];

学科分类号：

100201 [内科学]; 100221 [泌尿外科学];

摘要：

目的该研究观察在糖基化终末产物(AGEs)诱导大鼠肾小球系膜细胞(GMCs)中,心肌素相关转录因子MRTFA的变化及其对细胞黏附分子(ICAM-1)及纤维连接蛋白(FN)的影响,探讨MRTF-A是否通过影响NF-κB信号通路参与糖尿病肾病。方法 AGEs条件下,抑制剂CCG-1423抑制MRTF-A,或对MRTF-A进行过表达,干扰处理,通过Western blot检测MRTF-A、ICAM-1和FN的蛋白水平变化及p65在细胞核内的水平。结果在AGEs诱导的GMCs中,MRTF-A的蛋白表达增加;干扰或抑制MRTF-A能够下调AGEs诱导的FN和ICAM-1的蛋白表达及p65入核水平;而过表达MRTF-A则进一步上调FN和ICAM-1的蛋白表达及p65入核水平。结论 AGEs能够上调GMCs中MRTF-A的表达,且MRTF-A通过影响NF-κB信号通路参与介导了AGEs诱导的FN和ICAM-1的蛋白表达。

引用

收藏

页码：572 / 577

页数：6

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胆汁酸膜受体TGR5对高糖培养的大鼠肾小球系膜细胞FN、TGF-β1的调控作用 [J].

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