rhG-CSF动员正常人外周血干细胞过程中对T淋巴细胞增殖的影响

被引：21

作者：

陈松鹤

黄晓军

机构：

[1] 北京大学人民医院,北京大学人民医院北京大学血液病研究所,北京大学血液病研究所

来源：

中华血液学杂志 | 2003年 / 08期

关键词：

粒细胞集落刺激因子; 抗原提呈细胞; T淋巴细胞;

D O I：

暂无

中图分类号：

R457.7 [骨髓移植];

学科分类号：

100215 ;

摘要：

目的　探讨rhG CSF体内对T淋巴细胞增殖影响的作用机制。方法　取行异基因造血干细胞移植的HLA相合的同胞供者应用rhG CSF前后的静脉血和单采物 ,用MTT法测定去除单核细胞前、后的外周血单个核细胞 (MNC)对PHA增殖反应的改变。用流式细胞术检测单核细胞绝对数的改变、抗原呈递细胞 (包括单核细胞和B淋巴细胞 )协同刺激因子CD80 (B7 1 )、CD86(B7 2 )的表达以及贴壁前、后CD1 4+细胞比例。结果　rhG CSF体内作用后 ,外周血中单核细胞绝对数升高了 (4.2 0± 1 .74)倍 ,T细胞在去除单核细胞后增殖能力部分恢复 ,但仍较G CSF作用前平均降低 2 0 .58%。单核细胞表达共刺激信号CD86下降了 (66 .96± 1 3 .87) % ,相对平均荧光强度降低了 (31 .31± 1 2 .91 ) % ,B淋巴细胞中表达CD80 降低了 (45 .77± 2 6 .58) %。结论　G CSF体内作用后 ,单核细胞数量的改变抑制T淋巴细胞增殖 ,但它并不能完全解释T淋巴细胞的反应低下 ,而单核细胞和B淋巴细胞表达低水平的B7分子 ,提示这些抗原提呈细胞功能的改变可能参与T淋巴细胞增殖改变

引用

页码：31 / 33

页数：3

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