扇贝异源四倍体诱导的初步研究

被引：4

作者：

迟长凤 ^{[1
,2
]}

杨爱国 ^{[1
]}

王清印 ^{[1
]}

刘志鸿 ^{[1
]}

周丽青 ^{[1
]}

吴常文 ^{[2
]}

机构：

[1] 中国水产科学研究院黄海水产研究所

[2] 浙江海洋学院海洋科学学院

来源：

水产学报 | 2009年 / 33卷 / 01期

关键词：

栉孔扇贝; 虾夷扇贝; 异源四倍体; 流式细胞术;

D O I：

暂无

中图分类号：

S917.4 [水产动物学];

学科分类号：

0908 ;

摘要：

The allotetraploid in cross-fertilized eggs of Chlamys farreri♀× Patinopecten yessoensis♂ was induced in this paper.In order to find out the appropriate duration time of 6-DMAP treatment,ploidy status of induction offspring was analyzed by flow cytometry.The results showed that the optimal duration time of 6-DMAP treatment was to treat the fertilized eggs with 50 mg/L 6-DMAP for 15 min based on the integrated evaluation of the results of fertilization rate,survival rate of D-larvae,development status of larvae,and percentage of tetraploid.The results of flow cytometry showed that 17.3% tetraploid was obtained which showed that allotetraploid could be gotten with this method.The causes of the problems in the work were also discussed in this paper.

引用

页码：165 / 170

页数：6

共 13 条

[1]

栉孔扇贝四倍体诱导机理的初步研究.[D].李永仁.中国科学院研究生院（海洋研究所）.2005, 04

[2]

中国主要海产贝类健康养殖技术.[M].刘世禄;杨爱国主编;.海洋出版社.2005,

[3] 抑制栉孔扇贝第一极体对受精卵染色体行为及胚胎倍性组成的影响 [J].