脑内移植脑源性神经营养因子载体细胞对新生大鼠缺氧缺血性脑损伤的保护作用

被引：17

作者：

洪新如

侍坚

郑铃

任榕娜

机构：

[1] 中国人民解放军南京军区福州总医院儿科,第二军医大学神经生物学教研室,福建医科大学基因工程室,中国人民解放军南京军区福州总医院儿科,

来源：

中华儿科杂志 | 2002年 / 03期

关键词：

脑源性神经营养因子; 脑缺氧; 脑缺血; 大鼠; 细胞移植;

D O I：

暂无

中图分类号：

R722.121 [];

学科分类号：

100202 ;

摘要：

目的　观察脑内移植脑源性神经营养因子 (BDNF)载体细胞对新生大鼠缺氧缺血性脑损伤的疗效。方法　 7日龄新生大鼠随机分为三组 :缺氧缺血 +对照细胞移植组 (A组 )、缺氧缺血 +BDNF载体细胞移植组 (B组 )和假手术组 (C组 )。A、B组分别在缺氧缺血性脑损伤后即刻脑内植入不含BDNF的对照细胞和构建的BDNF载体细胞 ,C组于假手术后相应时间注射等量载液 ,48h观察脑含水量及细胞凋亡的变化 ;2 1d测定脑重量 ,并观察脑大体、脑区和神经细胞的损伤状况。　结果(1)脑缺氧缺血后 48h ,左侧大脑含水量A、B组均显著高于C组 ,B组 [(88 4± 1 4) % ]显著低于A组[(89 9± 1 5 ) % ](P <0 0 1) ;细胞凋亡检测表明 ,B组左侧皮层、海马和纹状体细胞凋亡率 [分别为(2 1 4± 4 8) %、(2 4 6± 4 8) %和 (2 7 1± 5 1) % ]均非常显著低于A组 [分别为 (4 4 3± 6 6 ) %、(4 9 6±8 7) %和 (38 9± 6 3) % ](P <0 0 1)。 (2 )缺氧缺血后 2 1d ,A、B组左侧大脑半球明显萎缩 ,其重量均低于C组 ;B组左脑萎缩比A组减轻 15 6 % ,差异有显著性 (F =2 2 148,P <0 0 1)。 (3)缺氧缺血后2 1d ,B组左右侧皮层、海马和纹状体体积比 [分别为 (88± 10 ) %、(79± 12 ) %和 (75± 10 ) % ]显著高于A组 [分别为 (4 5± 8) %、(35± 5

引用

页码：39 / 43

页数：5

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