隐甲藻深层培养产生二十碳五烯酸的研究

The effects of the incubation temperature,initial pH of the medium,carbon source and nitrogen source on the production of Crypthecodinium cohnii C98 were studied.Through orthogonal experiments,the optimum culture medium was obtained (g/L):Glucose,65;Yeast extract,2.0;KNO 3 3.0,KH 2PO 4 1.0,MgSO 4·7H 2O 0.6,NaCl 5.8,CaCl 2·2H 2O 0.1,ZnSO 4·7H 2O 0.0067,FeCl 3·6H 2O 0.014,CuSO 4·5H 2O 0.0004,MnSO 4·H 2O 0.0002.Under the optimum culture conditions,the microalgae dry cell weight and eicosapentaenoic acid was 18.3g/L and 0.891 g/L,respectively.The submersion culture process was analysed.