目的:建立RP-HPLC法测定垂盆草药材中槲皮素、山柰素和异鼠李素的含量。方法:采用Thermo BDS C18(4.6mm×150mm,5μm)色谱柱,以甲醇-0.4%磷酸(45∶55)为流动相;检测波长为360nm。结果:槲皮素、山柰素和异鼠李素分别在0.09558~0.6690μg,0.01974~0.1382μg和0.0206~0.1442μg范围内峰面积与进样量呈良好的线性关系,r分别为0.9999,0.9998,0.9999;平均回收率分别为98.22%,96.44%,96.50%。结论:该法简便可行,结果准确可靠,适用于垂盆草药材的质量控制。