毕赤氏酵母工程菌高密度发酵表达恶性疟原虫融合抗原的研究

被引：4

作者：

郭美锦

吴康华

储炬

庄英萍

张嗣良

黄大庆

张青锋

潘卫庆

机构：

[1] 华东理工大学生物反应器工程国家重点实验室!上海

[2] 江西农业大学生物工程系

[3] 南昌

[4] 华东理工大学

来源：

生物工程学报 | 2001年 / 04期

关键词：

甲醇营养型毕赤酵母; 恶性疟原虫融合抗原; 发酵条件优化; 高密度发酵;

D O I：

10.13345/j.cjb.2001.04.024

中图分类号：

R186 [预防接种、计划免疫、药物预防];

学科分类号：

100401 ;

摘要：

The effects of cultural conditions,which were fermentation period when methanol was as sole carbon source,methanol concentration,range of pH,on the expression of the chimeric protein of Plasmodium falciparum in genetically engineered methylotrophic Pichia pastoris were investigated by shake flask experiments in this paper.The results showed:(1)fermentation period with methanol inducement is about 96 hours;(2)the optimum methanol concentration as sole carbon source is 10g/L;(3)the range of pH is from 6.0 to 7.0. On the base of above experimental results the cell high-density fermentation had been done on the FMG-5L fermentor with multi-sensors.The results showed that the cell optical density (OD 600) can reached 550 and the ma-ximum expression level of target proteins was 780mg/L which was 4 times higher or more than the shake flask culture's.

引用

页码：456 / 459

页数：4