具核梭杆菌相关细菌生物膜促进巨噬细胞M2型极化和结肠癌化疗耐药的研究

被引：10

作者：

陆诗媛 ^{[1
]}

洪洁 ^{[1
]}

陈萦晅 ^{[1
]}

陈锦先 ^{[2
]}

钟鸣 ^{[2
]}

房静远 ^{[1
]}

机构：

[1] 上海交通大学医学院附属仁济医院消化科上海市消化疾病研究所

[2] 上海交通大学医学院附属仁济医院胃肠外科

来源：

上海交通大学学报(医学版) | 2020年 / 40卷 / 08期

关键词：

细菌生物膜; 具核梭杆菌; 肿瘤相关巨噬细胞; 结肠癌; 化疗; 耐药;

D O I：

暂无

中图分类号：

R735.35 [];

学科分类号：

摘要：

目的·探索具核梭杆菌(Fusobacterium nucleatum,F.n)相关细菌生物膜对结肠癌浸润的肿瘤相关巨噬细胞(tumor-associated macrophages,TAMs)极化和化疗耐药的影响。方法·培养并收集液基和生物膜来源的F.n上清干预肠癌细胞或巨噬细胞。采用实时荧光定量PCR检测各组肠癌细胞耐药相关基因表达水平,采用CCK8试剂盒检测化疗药物对各组肠癌细胞生长抑制率,采用实时荧光定量PCR和免疫细胞化学染色检测巨噬细胞极化分型。采用荧光原位杂交和免疫化学染色检测结肠癌患者组织标本生物膜状态和TAMs浸润分型情况。结果·生物膜来源的F.n培养上清上调肠癌细胞耐药基因(如MDR1/Abcb1a/Abcb1b)表达更为明显,化疗药物处理后细胞的生长抑制率更低。生物膜来源的培养上清更能促进巨噬细胞M2型极化。术后化疗复发的结肠癌组织中细菌生物膜阳性率和M2型巨噬细胞比例更高。结论·F.n相关细菌生物膜促进肿瘤相关巨噬细胞M2型极化和结肠癌化疗耐药,影响结肠癌患者预后。

引用

页码：1018 / 1027

页数：10

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