芥菜型油菜与甘蓝型油菜种间杂种后代的RAPD分析

被引:11
作者
刘忠松
官春云
陈社员
李栒
Tom Osborn
不详
机构
[1] 湖南农业大学
[2] University of Wisconsin
[3] Madison
[4] WI
[5] USA 长沙
[6] 长沙
[7] 长沙
关键词
甘蓝型油菜; 芥菜型油菜; 种间杂交; RAPD分析; 遗传;
D O I
暂无
中图分类号
S565.4 [油菜籽(芸薹)];
学科分类号
摘要
为了揭示芥菜型油菜与甘蓝型油菜种间杂种后代的遗传分离规律 ,用芥菜型油菜四川黄籽作母本与甘蓝型油菜 10 47自交系杂交 ,杂种F1人工自交或用 10 47自交系花粉回交 ,然后将亲本、杂种F1、F2 和BC1代用 16个随机引物进行PCR扩增。共扩增出 98条带 ,其中双亲共有的带 35条 ,四川黄籽特异带 30条 ,10 47特异带 33条。带型统计分析结果表明在芥甘杂种后代中来自 10 47的遗传物质比来自四川黄籽的遗传物质更能传递给后代 ,这种趋势与杂种后代植株的育性无关 ,在回交后代中四川黄籽的遗传物质比在自交后代中丢失更快。并对造成芥甘种间杂种后代分离扭曲的原因进行了探讨。
引用
收藏
页码:1010 / 1015
页数:6
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