IL-21调节外周血中Treg细胞的表达在Graves病发病机制中的研究

被引：19

作者：

黄小庆

刘纯

机构：

[1] 重庆医科大学附属第一医院内分泌科

来源：

中国免疫学杂志 | 2016年 / 32卷 / 06期

关键词：

IL-21; Graves病; Treg细胞; Foxp3; IL-10;

D O I：

暂无

中图分类号：

R581.1 [甲状腺机能亢进症];

学科分类号：

100201 [内科学];

摘要：

目的:探究IL-21调节外周血Treg细胞的表达在Graves病发病机制中的研究。方法:收集28例初发Graves病患者(GD)、27例经药物治疗后甲状腺功能恢复正常的GD(e GD)患者和24例健康对照者(NC),电化学发光法检测患者的血清FT3、FT4、u TSH、Tg Ab、TPOAb和TRAb水平。分离研究对象外周血单个核细胞分为IL-21刺激组与未刺激组。实时荧光定量PCR法检测Foxp3和IL-10 mRNA的表达水平;ELISA法检测培养上清液中IL-10蛋白的表达。结果:GD组甲功和自身抗体水平与e GD组和NC组比较,差异均有统计学意义(P<0.05);但e GD组甲功和自身抗体水平与NC组比较差异无统计学意义(P>0.05)。IL-21刺激前,GD组的Foxp3、IL-10 mRNA和IL-10蛋白表达水平均较e GD组和NC组明显升高,差异有统计学意义(P<0.05),但e GD组与NC组比较差异无统计学意义(P>0.05)。经IL-21刺激后,GD组的Foxp3、IL-10 mRNA和IL-10蛋白的表达水平均显著低于刺激前,差异有统计学意义(P<0.05)。结论:IL-21可能通过抑制Treg细胞的分化及其效应分子IL-10的产生,使Treg细胞数量和功能下降,降低其对效应T细胞的抑制能力,从而参与GD的发病过程。

引用

收藏

页码：853 / 857+862 +862

页数：6

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共 18 条

[1]

IL-21上调IL-17的表达在Graves病中的意义 [J].

卢永霞 ;

刘纯 .

上海交通大学学报(医学版), 2014, 34 (01) :65-69

[2]

Increased IL-21/IL-21R expression and its proinflammatory effects in autoimmune thyroid disease [J].

Guan, Li-juan ;

Wang, Xuan ;

Meng, Shuai ;

Shi, Liang-feng ;

Jiang, Wen-juan ;

Xiao, Ling ;

Shi, Xiao-hong ;

Xu, Jian ;

Zhang, Jin-an .

CYTOKINE, 2015, 72 (02) :160-165

[3]

Regulatory T cells in vitiligo: Implications for pathogenesis and therapeutics [J].

Dwivedi, Mitesh ;

Kemp, E. Helen ;

Laddha, Naresh C. ;

Mansuri, Mohmmad Shoab ;

Weetman, Anthony P. ;

Begum, Rasheedunnisa .

AUTOIMMUNITY REVIEWS, 2015, 14 (01) :49-56

[4]

Th17 and regulatory T cell balance in autoimmune and inflammatory diseases [J].

Noack, Melissa ;

Miossec, Pierre .

AUTOIMMUNITY REVIEWS, 2014, 13 (06) :668-677

[5]

Peripheral CD4+cell prevalence and pleuropulmonary manifestations in systemic lupus erythematosus patients [J].

Vincze, Krisztina ;

Kovats, Zsuzsanna ;

Cseh, Aron ;

Pasti, Krisztina ;

Kiss, Emese ;

Polgar, Anna ;

Vasarhelyi, Barna ;

Szabo, Attila J. ;

Bohacs, Aniko ;

Tamasi, Lilla ;

Losonczy, Gyoergy ;

Mueller, Veronika .

RESPIRATORY MEDICINE, 2014, 108 (05) :766-774

[6]

Assessment of intracellular cytokines and regulatory cells in patients with autoimmune diseases and primary immunodeficiencies - Novel tool for diagnostics and patient follow-up [J].

Osnes, Liv T. ;

Nakken, Britt ;

Bodolay, Edit ;

Szodoray, Peter .

AUTOIMMUNITY REVIEWS, 2013, 12 (10) :967-971

[7]

Impairment of regulatory T cells in myasthenia gravis: Studies in an experimental model [J].

Gertel-Lapter, Smadar ;

Mizrachi, Keren ;

Berrih-Aknin, Sonia ;

Fuchs, Sara ;

Souroujon, Miriam C. .

AUTOIMMUNITY REVIEWS, 2013, 12 (09) :894-903

[8]

Loss of the balance between CD4+Foxp3+ regulatory T cells and CD4+IL17A+ Th17 cells in patients with type 1 diabetes [J].

Ryba-Stanislawowska, Monika ;

Skrzypkowska, Maria ;

Mysliwiec, Malorzata ;

Mysliwska, Jolanta .

HUMAN IMMUNOLOGY, 2013, 74 (06) :701-707

[9]

The activation and regulation of IL-17 receptor mediated signaling [J].

Song, Xinyang ;

Qian, Youcun .

CYTOKINE, 2013, 62 (02) :175-182

[10]

Th17 and regulatory T lymphocytes in primary biliary cirrhosis and systemic sclerosis as models of autoimmune fibrotic diseases [J].

Fenoglio, Daniela ;

Bernuzzi, Francesca ;

Battaglia, Florinda ;

Parodi, Alessia ;

Kalli, Francesca ;

Negrini, Simone ;

De Palma, Raffaele ;

Invernizzi, Pietro ;

Filaci, Gilberto .

AUTOIMMUNITY REVIEWS, 2012, 12 (02) :300-304