封闭ERCC1基因表达对卵巢癌细胞耐药的影响

被引：12

作者：

李体远

Jing Jie Yu

Eddie Reed

机构：

[1] 暨南大学医学院附二院深圳市人民医院临床医学研究中心

[2] West Virginia University

[3] Mary Babb Randolph Cancer Center

[4] Morgantown

[5] West Virginia -

[6] West Virginia - 深圳

来源：

中国肿瘤生物治疗杂志 | 2004年 / 02期

关键词：

ERCC1/DNA损伤修复; 耐药/顺铂; 卵巢癌;

D O I：

暂无

中图分类号：

R737.31 [卵巢肿瘤];

学科分类号：

100214 ;

摘要：

目的:探讨通过封闭ERCC1基因表达干扰DNA损伤修复路径及其对卵巢癌细胞对铂类药物耐药性的影响。方法:构建ERCC1基因反义表达载体,转染人卵巢癌细胞A2780及A2780/CF70,采用Northern杂交及Western Blot分析人卵巢癌细胞内ERCC1基因RNA及蛋白表达水平变化;利用荧光素酶报告基因系统观察宿主细胞对DNA损伤的修复作用;采用MTT实验研究细胞对铂类药物耐药性的影响。结果:卵巢癌细胞转染反义ERCC1基因后,ERCC1基因转录水平、蛋白表达水平明显下降。荧光素酶报告基因系统结果证明,宿主细胞的DNA修复活性下降。MTT实验表明,伴随ERCC1基因表达水平下降,细胞对顺铂的耐药性降低。结论:转染ERCC1反义基因显著影响宿主细胞的核苷酸切除修复,影响细胞对顺铂的耐药性。

引用

页码：92 / 95

页数：4

共 9 条

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