人和大鼠自噬相关新基因WIPI-3的电子克隆和序列分析

被引：8

作者：

王海涛 ^{[1
]}

畅继武 ^{[1
]}

马小兵 ^{[2
]}

张一兵 ^{[1
]}

韩瑞发 ^{[1
]}

马富玲 ^{[1
]}

张淑敏 ^{[1
]}

机构：

[1] 天津医科大学第二医院

[2] 华北煤炭医学院病理教研室

来源：

现代生物医学进展 | 2006年 / 04期

关键词：

WIPI-3; 电子克隆; 自噬; 生物信息学; 比较基因组;

D O I：

10.13241/j.cnki.pmb.2006.04.006

中图分类号：

R346 [];

学科分类号：

摘要：

目的:人和大鼠WIPI-3基因的电子克隆和序列分析。方法:综合运用基因电子拼接和比较基因组分析等生物信息学手段进行新基因的电子克隆和注释。通过拼接CR593190、NM019613和BC000974 cDNA序列获得人WIPI-3 cDNA全长序列,通过拼接EST序列CB727439、CB737031、BF557312、BG663387和预测的CDS获得大鼠WIPI3基因的cDNA序列。结果:成功克隆了人和大鼠自噬相关新基因WIPI-3的cDNA,上述两个新基因序列均得到了人类基因组序列和EST序列的双重支持,另外经RT-PCR验证电子克隆的基因序列也是正确的,而且序列均已被GenBank收录,其编号分别为:AM182326和NM001039587。其中,人WIPI-3基因修正了目前基因库中注释的WIPI-3序列,而大鼠基因则是国际上首次克隆,并被确定为参考序列。结论:基因电子拼接结合种属同源基因比较分析,可大大提高电子克隆的精确性,这种方法可有效用于新基因的克隆和注释。

引用

页码：16 / 21

页数：6

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