环糊精超分子体系的手性识别研究──D,L-色氨酸对映体的手性识别荧光分析法

被引:12
作者
谢剑炜
翟言强
杨造萍
阮金秀
机构
[1] 中国军事医学科学院毒物药物研究所!北京,
关键词
手性识别; 环糊精; 荧光法; 色氨酸对映体;
D O I
暂无
中图分类号
O636 [天然高分子化合物(高聚物)];
学科分类号
摘要
The paper reports the simultaneous fluorescence determination of D,L-tryptophanenantiomer by chiral recognition of β-cyclodextrin for the first time. Chiral discrimination isobserved for fluorescence emission of D- and L-tryptophan when complexed toβ-cyclodextrin, and is significantly related to reaction temperature, time and PH. The proposedenantiomeric resolution of D- and L-tryptophan is based on the non-fluorescellce emission ofLtryptophan in β-cyclodextrln solution at 35℃ and after 24 h standing by. The fluorescenceemission and absorbance of L-tryptophan disappear with the Increase of β-cyclodextrin at 35℃ and 24 h standing by, where as it is not observed for D-tryptophan. The detection limitsare 3.1×10-8 mol/L for D-tryptophan and 2.7 ×10-7 mol/L for L-tryptophan, respectively,with a RSD of 1. 0%-2. 6% (n=7). The method has been shown to be accurate with a ratioof 2%(molar ratio) of one enantiomer in the presence of the other. The observed pseudofirst-order rate constant is calculated. The reaction mechanism has been discussed usingpyrene as a fluorescence probe.
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页数:3
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[1]  
Schneider H.J. Angew.Chem.Int.Ed.Engl . 1991