转化生长因子-β1对新生大鼠星形胶质细胞结缔组织生长因子表达的影响

被引：1

作者：

李彤 ^{[1
]}

袁彬 ^{[2
]}

邹飞 ^{[1
]}

机构：

[1] 南方医科大学公共卫生与热带医学学院

[2] 新乡医学院第一附属医院神经内科

来源：

郑州大学学报(医学版) | 2008年 / 01期

关键词：

转化生长因子-β1; 结缔组织生长因子; 星形胶质细胞; 大鼠;

D O I：

10.13705/j.issn.1671-6825.2008.01.048

中图分类号：

R741 [神经病学];

学科分类号：

1002 ;

摘要：

目的:研究转化生长因子-β1(TGF-β1)对新生大鼠大脑皮层星形胶质细胞表达结缔组织生长因子(CT-GF)的影响。方法:体外培养的新生SD大鼠大脑皮层星形胶质细胞分为对照组:无血清培养基(FSM)培养;实验组:分别用含1μg/L,2μg/L,4μg/LTGF-β1的FSM培养,24h后,分别用RT-PCR和Western Blot检测2组细胞中CTGF mRNA和蛋白的表达。结果:对照组CTGFmRNA和蛋白表达均为阴性;实验组CTGF mRNA和蛋白表达皆呈阳性,且随着TGF-β1质量浓度的增加,2者的表达量有增高的趋势(F=80.889和200.300,P均<0.01)。结论:TGF-β1能上调星形胶质细胞中CTGF的表达。

引用

页码：47 / 50

页数：4

共 5 条

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