Antiviral Activity of GuiQi Polysaccharides against Enterovirus 71 in vitro

被引：9

作者：

Xiuying Pu ^{[1
]}

Hengrui Wang ^{[1
]}

Yan Li ^{[2
]}

Wenbo Fan ^{[1
]}

Shuang Yu ^{[1
]}

机构：

[1] School of Life Science and Engineering,Lanzhou University of Technology

[2] Shanghai Hengrui Pharmaceutical Limited Company

来源：

Virologica Sinica | 2013年 / 28卷 / 06期

关键词：

D O I：

暂无

中图分类号：

R373 [人体病毒学（致病病毒）];

学科分类号：

100123 [人体微生态学];

摘要：

In this study,we have investigated the antiviral activity of GuiQi polysaccharides (GQP) upon enterovirus 71 (EV71) in vitro.An assay using methyl thiazolyl tetrazolium (MTT),and analyses of cytopathic effects (CPE)were used to examine the antiviral activity of GQP upon Vero cells infected with EV71.The results revealed that GQP at concentrations below 31.2μg/mL exhibited significant antiviral effects upon EV71 when applied under three different experimental protocols.GQP was most strongly active in preventing the adsorption of EV71 to target cells and in this respect it was significantly more effective than ribavirin.In addition,it was clear that GQP could inhibit viral replication when added to cells 2 h after infection,but if added at the point of infection its effect was weak.GQP is considered to be less toxic than ribavirin,and may warrant further evaluation as a possible agent in the treatment of hand,foot and mouth disease (HFMD).

引用

页码：352 / 359

页数：8

共 12 条

[1]

Comparison of Clinical Features Between Coxsackievirus A2 and Enterovirus 71 During the Enterovirus Outbreak in Taiwan, 2008: A Children's Hospital Experience [J].

Chen, Shih-Perng ;

Huang, Yhu-Chering ;

Li, Wen-Chen ;

Chiu, Cheng-Hsun ;

Huang, Chung-Guei ;

Tsao, Kuo-Chien ;

Lin, Tzou-Yien .

JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION, 2010, 43 (02) :99-104

[2]

Virology, epidemiology, pathogenesis, and control of enterovirus 71 [J].

Solomon, Tom ;

Lewthwaite, Penny ;

Perera, David ;

Cardoso, Mary Jane ;

McMinn, Peter ;

Ooi, Mong How .

LANCET INFECTIOUS DISEASES, 2010, 10 (11) :778-790

[3]

Developments towards antiviral therapies against enterovirus 71.[J].Kan X. Wu;Mary M.-L. Ng;Justin J.H. Chu.Drug Discovery Today.2010, 23

[4]

Antioxidant activities of polysaccharides from Hyriopsis cumingii [J].

Qiao, Deliang ;

Ke, Chunling ;

Hu, Bing ;

Luo, Jianguang ;

Ye, Hong ;

Sun, Yi ;

Yan, Xiaoyan ;

Zeng, Xiaoxiong .

CARBOHYDRATE POLYMERS, 2009, 78 (02) :199-204

[5]

Extraction, characterization of Astragalus polysaccharides and its immune modulating activities in rats with gastric cancer [J].

Li Rui ;

Chen Wei-chang ;

Wang Wei-peng ;

Tian Wen-yan ;

Zhang Xue-guang .

CARBOHYDRATE POLYMERS, 2009, 78 (04) :738-742

[6]

Purification; structure and immunobiological activity of a water-soluble polysaccharide from the fruiting body of Pleurotus ostreatus.[J].Yongxu Sun;Jicheng Liu.Bioresource Technology.2008, 2

[7]

Structural characterization of a water-soluble polysaccharide from the roots of Codonopsis pilosula and its immunity activity [J].

Sun Yongxu ;

Liu Jicheng .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2008, 43 (03) :279-282

[8]

Purification; structure and immunobiological activity of a new water-soluble polysaccharide from the mycelium of Polyporus albicans (Imaz.) Teng.[J].Yongxu Sun;Shusheng Wang;Tianbao Li;Xia Li;Lili Jiao;Liping Zhang.Bioresource Technology.2007, 4

[9]

Survival after pulmonary edema due to enterovirus 71 encephalitis [J].