陆地棉CC-NBS-LRR基因的克隆及特征分析

被引：7

作者：

张保龙

杨郁文

倪万潮

佘建明

沈新莲

何晓兰

张香桂

徐英俊

姚姝

机构：

[1] 江苏省农业科学院农业生物遗传生理研究所

来源：

江苏农业学报 | 2006年 / 04期

关键词：

陆地棉; CC-NBS-LRR基因; GHNBS; 基因表达;

D O I：

暂无

中图分类号：

S562 [棉];

学科分类号：

摘要：

根据抗病基因核苷酸结合位点(Nuc leotide b ind ing site,NBS)设计简并性引物,从陆地棉cDNA中进行RT-PCR扩增。获得含NBS保守域的EST,进一步用RACE技术和TAIL PCR技术获得其中1个EST的全长基因序列,并获得GHNBS基因的5′调控序列。此基因被命名为GHNBS。该基因的编码区长2 583 bp,编码861个氨基酸,GHNBS编码的氨基酸序列与拟南芥R基因具有28%的同源性。GHNBS与拟南芥的其他几个NBS-LRR基因比较发现,它们在保守区外的相似性相当低。Southern杂交和网上数据库搜索分析都表明GHNBS是1个寡拷贝基因。通过半定量RT-PCR分析发现GHNBS在棉花的蕾、花瓣、韧皮部、根及叶中均有表达且在根、叶中表达量比其它部位强,在木质部基本不表达。

引用

页码：351 / 355

页数：5

共 7 条

[1]

The promoter of the nematode resistance gene Hs1 pro?1 activates a nematode-responsive and feeding site-specific gene expression in sugar beet ( Beta vulgaris L.) and Arabidopsis thaliana[J] . Tim Thurau,Sirak Kifle,Christian Jung,Daguang Cai. Plant Molecular Biology . 2003 (3)

[2]

Identification of resistance gene analogs in cotton ( Gossypium hirsutum L.)[J] . H. Tan,F.E. Callahan,X.-D. Zhang,M. Karaca,S. Saha,J.N. Jenkins,R.G. Creech,D.-P. Ma. Euphytica . 2003 (1)

[3]

Resistance gene analogues of chickpea (Cicer arietinum L.): isolation, genetic mapping and association with a Fusarium resistance gene cluster[J] . B. Huettel,D. Santra,F. Muehlbauer,G. Kahl. Theoretical and Applied Genetics . 2002 (2-3)

[4] NBS-LRR sequence family is associated with leaf and stripe rust resistance on the end of homoeologous chromosome group 1S of wheat [J].

Spielmeyer, W ;

Huang, L ;

Bariana, H ;

Laroche, A ;

Gill, BS ;

Lagudah, ES .

THEORETICAL AND APPLIED GENETICS, 2000, 101 (07) :1139-1144

[5]

Resistance gene analogues from rice: cloning, sequencing and mapping[J] . R. Mago,S. Nair,M. Mohan. TAG Theoretical and Applied Genetics . 1999 (1-2)

[6]

Cloning and characterisation of a family of disease resistance gene analogs from wheat and barley[J] . S. Seah,K. Sivasithamparam,A. Karakousis,E. S. Lagudah. TAG Theoretical and Applied Genetics . 1998 (5-6)

[7]

Characterization of disease resistance gene-like sequences in near-isogenic lines of tomato[J] . T. Ohmori,M. Murata,F. Motoyoshi. TAG Theoretical and Applied Genetics . 1998 (3-4)

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