转化生长因子-β1通过促进结缔组织生长因子表达抑制胰腺癌细胞PANC-1增殖

被引：3

作者：

苏燕 ^{[1
]}

李宏伟 ^{[2
]}

崔培林 ^{[2
]}

王春玲 ^{[3
]}

修瑞娟 ^{[2
]}

机构：

[1] 包头医学院生物化学与分子生物学教研室

[2] 中国协和医科大学中国医学科学院微循环研究所

[3] 煤炭总医院心脏中心

来源：

现代生物医学进展 | 2007年 / 12期

关键词：

转化生长因子-β1; 结缔组织生长因子; 胰腺癌; 增殖; 凋亡;

D O I：

10.13241/j.cnki.pmb.2007.12.032

中图分类号：

R735.9 [胰腺肿瘤];

学科分类号：

摘要：

目的:研究转化生长因子-β1(Transforming growth factor-β1,TGF-β1)和结缔组织生长因子(connective tissue growth factor,CTGF)对人胰腺癌细胞系PANC-1增殖的影响。方法:首先利用MTT检测重组TGF-β1、CTGF及anti-CTGF抗体处理PANC-1细胞后,细胞的增殖状态,用流式细胞仪(Flow cytometer,FCM)检测转染pcDNA3.1(-)-CTGF后PANC-1细胞的凋亡情况,最后利用RT-PCR检测TGF-β1作用PANC-1细胞后CTGF mRNA的表达。结果:TGF-β1和CTGF均呈浓度依赖性抑制PANC-1细胞增殖,加入anti-CTGF抗体可以取消TGF-β1对PANC-1细胞增殖的抑制作用;FCM结果显示pcDNA3.1(-)-CTGF质粒转染促进PANC-1细胞凋亡;RT-PCR则证明TGF-β1促进PANC-1细胞中CTGF mRNA表达。结论:TGF-β1可能通过促进CTGF表达来发挥抑制胰腺癌细胞PANC-1增殖的作用。

引用

页码：1761 / 1764

页数：4

共 17 条

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